San-Ao Decoction () Regulates Urine Volume on Bronchial Asthma Model Mice.
10.1007/s11655-018-3006-2
- Author:
Min WANG
1
;
Yan SUN
1
;
Shu-Jing ZHANG
1
;
Yu-Shan GAO
1
;
Ya-Nan WEI
1
;
Peng-Na ZHAO
1
;
Miao LIU
1
;
Jia-Min YANG
1
;
Feng-Jie ZHENG
1
;
Hong XU
1
;
Yu-Hang LI
2
Author Information
1. School of Chinese Medicine, Beijing University of Chinese Medicine, Beijing, 100029, China.
2. School of Chinese Medicine, Beijing University of Chinese Medicine, Beijing, 100029, China. liyuhang@bucm.edu.cn.
- Publication Type:Journal Article
- Keywords:
San-Ao Decoction;
aquaporin;
bronchial asthma;
regulation of fluid passage;
urine volume
- From:
Chinese journal of integrative medicine
2021;27(3):212-219
- CountryChina
- Language:English
-
Abstract:
OBJECTIVE:To observe the effect of San-Ao Decoction (, SAD) on water metabolism of bronchial asthra model mice.
METHODS:Forty-five female BALB/c mice were randomly divided into control, model and SAD groups by a random number table, 15 mice in each group. A composite method with ovalbumin (OVA) sensitization and challenge was developed to establish bronchial asthma model. Mice in the control group were intraperitoneally injected with distilled water without aerosol inhalation challenge. On day 15-22, 0.3 mL SAD was administered via gastric route in SAD group, one time per day, while an equivalent volume of normal saline was used for gastric administration in the control and model groups. Changes in airway resistance in the inspiratory phase (RI-R-Area) were detected using an AniRes2005 system, and 5-h urine output was collected by metabolic cages. Histopathological changes in lung and kidney were observed by hematoxylin-eosin staining. mRNA expressions of aquaporin (AQP) 1 and AQP2 in kidney were detected by reverse transcription-polymerase chain reaction, and the protein expressions of AQP1 and AQP2 in kidney were detected by immunohistochemistry. Enzyme-linked immune sorbent assay was used to detect the OVA-specific endothelium-1 (ET-1), antidiuretic hormone (ADH), atrial natriuretic peptide (ANP), prostaglandin E
RESULTS:Compared with the control group, the serum IgE level in model group increased (P<0.01). Following the pathologic changes in lung tissue, no significant change in kidney tissue was observed among 3 groups. Compared with the control group, the mice in the model group showed elevated airway resistance during inhalation phase, higher mRNA and protein expression levels on AQP1 and AQP2 in kidney tissue and higher ET-1 levels in serum, lung and kidney tissues, ADH and ANP in lung and serum, PGE
CONCLUSION:San-Ao Decoction can regulate the urine volume through regulating AQP1 and AQP2 expression, and the expression of these in the kidneys might be regulated by ET-1, NO and Ang II.
