Effect of Competitive Antagonist of Transmembrane Glutamine Flux V-9302 on Apoptosis of Acute Myeloid Leukemia Cell Lines HL-60 and KG-1.
10.19746/j.cnki.issn.1009-2137.2021.03.005
- Author:
Qian-Qin LI
1
;
Shi-Yi PAN
1
;
Qian-Yi CHEN
2
;
Wei ZHOU
1
;
Shun-Qing WANG
3
Author Information
1. Department of Hematology, School of Medicine, South China University of Technology, Guangzhou 510006, Guangdong Province,China.
2. Guangzhou Medical University, Guangzhou 511436, Guangdong Province, China.
3. Department of Hematology, School of Medicine, South China University of Technology, Guangzhou 510006, Guangdong Province,China,E-mail: eywangshq@scut.edu.cn.
- Publication Type:Journal Article
- MeSH:
Apoptosis;
Cell Proliferation;
Glutamine;
HL-60 Cells;
Humans;
Leukemia, Myeloid, Acute
- From:
Journal of Experimental Hematology
2021;29(3):685-689
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the effect of V-9302 (an antagonist of transmembrane glutamine flux) on the proliferation and apoptosis of acute myeloid leukemia cells HL-60 and KG-1.
METHODS:HL-60 and KG-1 cells at logarithmic phase were treated by different concentrations of V-9302. CCK-8 assay was used to detect the proliferation of the cells. Annexin V-FITC / PI double staining flow cytometry was used to detect the apoptosis of HL-60 and KG-1 cells. The expressions of BAX, BCL-2 and Caspase3 were detected by RT-qPCR and Western blot.
RESULTS:V-9302 could significantly inhibit the growth of HL-60 and KG-1 cells. The concentration of V-9302 at 10, 20 μmol/L could significantly promote the apoptosis of HL-60 and KG-1 cells(P<0.05). The results of apoptosis related gene detection showed that when V-9302 was applied to HL-60 and KG-1 cell lines at 10 and 20 μmol/L, the expression levels of Pro-apoptotic protein genes BAX and Caspase3 in HL-60 and KG-1 were significantly higher than those in control group (P<0.05), while the expression level of anti-apoptotic protein gene BCL-2 was significantly lower than that in the control group (P<0.05). The results of Western blot were basically consistent with that of RT-qPCR.
CONCLUSION:Competitive antagonist of transmembrane glutamine flux V-9302 can significantly promote the apoptosis of acute myeloid leukemia cell lines HL-60 and KG-1.
