Analysis of blood components of Yougui Yin in normal rats and rats with kidney deficiency caused by adenine based on UPLC-MS technology.
10.19540/j.cnki.cjcmm.20201015.201
- Author:
Yu-Hong SHI
1
;
Huan YANG
1
;
Hai-Feng RAN
1
;
Han CHEN
1
;
Ji-Fen ZHANG
1
;
Qiang XUE
2
;
Shan FENG
1
;
Xiao-Yu XU
3
Author Information
1. Chongqing Key Laboratory of New Drug Screening from Traditional Chinese Medicine, Pharmacology of Chinese Materia Medica-the Key Discipline Constructed by the State Administration of Traditional Chinese Medicine, College of Pharmaceutical Sciences & Chinese Medicine, Southwest University Chongqing 400715, China.
2. Chongqing Medical and Pharmaceutical College Chongqing 401331, China.
3. Chongqing Key Laboratory of New Drug Screening from Traditional Chinese Medicine, Pharmacology of Chinese Materia Medica-the Key Discipline Constructed by the State Administration of Traditional Chinese Medicine, College of Pharmaceutical Sciences & Chinese Medicine, Southwest University Chongqing 400715, China Southwest University Hospital Chongqing 400715, China.
- Publication Type:Journal Article
- Keywords:
MRM;
UPLC-Q-TOF-MS;
Yougui Yin;
blood component;
multivariate statistical analysis;
serum pharmacochemistry
- MeSH:
Adenine;
Animals;
Chromatography, High Pressure Liquid;
Chromatography, Liquid;
Drugs, Chinese Herbal/toxicity*;
Kidney;
Rats;
Tandem Mass Spectrometry;
Technology
- From:
China Journal of Chinese Materia Medica
2021;46(9):2287-2297
- CountryChina
- Language:Chinese
-
Abstract:
Based on the serum medicinal method, this study aims to investigate the migrating components of Yougui Yin in the blood after intragastric administration, and to provide reference for the basic research of its pharmacodynamics. The kidney deficiency rat model was replicated by adenine method. Normal rats and model rats were administered orally for a single gavage of Yougui Yin. The components in blood were rapidly analyzed and identified by ultra-high performance liquid chromatography/quadrupole time-of-flight mass spectrometry(UPLC-Q-TOF-MS) and multiple reaction monitoring(MRM), and the migrating components in blood of Yougui Yin were explored by multivariate statistical analysis. The results showed that there were 42 characteristic peaks in the plasma of normal rats by UPLC-Q-TOF-MS technology and 13 chemical components were identified, including 6 alkaloids, 2 flavonoids, 2 triterpenoid saponins, 1 iridoid, 1 phenylpropanoid and 1 monoterpenoid. There were 22 characteristic peaks in the plasma of kidney-deficiency rats, and 12 chemical components were identified, including 2 iridoids, 6 alkaloids, 2 flavonoids, 1 monoterpenoid and 1 triterpenoid saponin. Verbascoside, isoacteoside, acteoside, pinoresinoldiglucoside, loganin and morroniside were identified by MRM both in the plasma of normal rats and kidney-deficiency rats. Compared with 85 monomer components in Yougui Yin, 17 common prototype components were found by UPLC-MS in the plasma of normal rats and kidney deficiency rats, including verbascoside, isoacteoside, acteoside, rehmapicrogenin derived from Rehmanniae Radix Praeparata, pinoresinol diglucoside and geniposidic acid from Eucommiea Cortex, loganin and morroniside derived from Corni Fructus, mesaconine, benzoylmesaconine, benzoylaconitine, benzoylhypacoitine, mesaconitine, aconitine derived from Aconiti Lateralis Radix Praeparata, liquiritin, isoliquiritin and glycyrrhizic acid derived from Glycyrrhizae Radix et Rhizoma. Thirty-one metabolites of medicinal ingredients not found in the plasma of adenine-induced kidney deficiency rats were also detected in the plasma of normal rats. Twelve metabolites of medicinal materials not found in the plasma of normal rats were detected in the plasma of kidney deficiency rats. The results of the study provide reference for explaining the material basis and mechanism of Yougui Yin in the treatment of kidney deficiency.
