Analysis of material basis of Schisandrae Chinensis Fructus in different growth stages based on chromatography.
10.19540/j.cnki.cjcmm.20200729.202
- Author:
Ming-Jie SONG
1
;
Xuan TENG
1
;
Chang LIU
2
;
Zhi-Man LI
2
;
Yin-Shi SUN
2
Author Information
1. State Local Joint Engineering Research Center of Ginseng Breeding and Application, College of Chinese Medicinal Materials, Jilin Agricultural University Changchun 130118, China.
2. Institute of Special Animal and Plant Sciences,China Academy of Agricultural Sciences Changchun 130112, China.
- Publication Type:Journal Article
- Keywords:
GC-MS;
Schisandrae Chinensis Fructus;
UPLC-MS;
growth stage;
material basis
- MeSH:
Chromatography, Liquid;
Drugs, Chinese Herbal;
Fruit/chemistry*;
Lignans/analysis*;
Schisandra;
Tandem Mass Spectrometry
- From:
China Journal of Chinese Materia Medica
2021;46(9):2245-2253
- CountryChina
- Language:Chinese
-
Abstract:
Schisandrae Chinensis Fructus in six growth stages was taken as materials to study the species and content changes of material basis, which were detected by UPLC, GC and MS chromatography, including lignans, nucleosides, aroma components and fatty acids. The results showed that the texture, color and taste of Schisandrae Chinensis Fructus in six growth stages were different. On the material basis, 12 lignans were detected by UPLC-MS, and the content of total lignans was higher in the samples from late August to early September, among which the highest content of schisandrin was 0.67%±0.01%, followed by schizandrol B, angeloylgomisin H and schisandrin B, and the total content increased with the maturity of Schisandrae Chinensis Fructus. Thirteen kinds of nucleosides were detected by UPLC. The total nucleoside content was the highest in late July samples, in which the contents of uridine and guanosine were higher and decreased after maturity. Aroma components and fatty acids were identified by GC-MS. A total of 53 aroma components were detected and the highest total content was appeared in late August samples, of which ylangene was higher and bergamotene was followed. A total of 24 kinds of fatty acids were detected. The fruits matured basically in August, and the content of fatty acids in the samples was the highest, among which linoleic acid content was top the list and oleic acid was the second. To sum up, the maturity of Schisandra chinensis fruit is related to the content and variety of various material bases, and the growth period has different influences on the quality of Schisandrae Chinensis Fructus. Therefore, the appropriate harvesting time should be determined according to the change law of target components. The results of this study can provide reference for the quality evaluation of Schisandrae Chinensis Fructus material basis.
