Rapid chemome profiling of chemical components of Lonicerae Japonicae Flos using DI-MS/MS~(ALL).
10.19540/j.cnki.cjcmm.20210220.302
- Author:
Xing-Cheng GONG
1
;
Wen-Jing LIU
1
;
Li-Bo CAO
1
;
Juan YU
2
;
Dan-Dan SI
3
;
Jun LI
4
;
Peng-Fei TU
1
;
Jun LI
1
;
Yue-Lin SONG
1
Author Information
1. Modern Research Center for Traditional Chinese Medicine, School of Chinese Materia Medica, Beijing University of Chinese Medicine Beijing 100029, China.
2. Zhangzhou Pianzaihuang Parmaceutical Co., Ltd. Zhangzhou 363000, China.
3. SCIEX China Beijing 100015, China.
4. State Key Laboratory of Natural and Biomimetic Drugs, Pharmaceutical Sciences,Peking University Beijing 100191, China.
- Publication Type:Journal Article
- Keywords:
DI-MS/MS~(ALL);
Lonicerae Japonicae Flos;
chemical components;
data independent acquisition;
unit mass window
- MeSH:
Chromatography, High Pressure Liquid;
Drugs, Chinese Herbal;
Lonicera;
Plant Extracts;
Tandem Mass Spectrometry
- From:
China Journal of Chinese Materia Medica
2021;46(9):2220-2228
- CountryChina
- Language:Chinese
-
Abstract:
A new method of MS/MS~(ALL) was designed to sequentially record a MS~2 spectrum at each unit mass window through gas phase fractionation concept, so as to offer an opportunity for universal MS~2 spectral recording with direct infusion(DI). As a proof-of-concept, DI-MS/MS~(ALL) was applied for rapid chemome profiling of a famous herbal medicine named Lonicerae Japonicae Flos. After each MS~2 spectrum was correlated to its precursor ion, the structural annotation was conducted by applying well-defined mass cracking rules, matching the mass spectral data with literatures and referring to those accessible databases. As a result, a total of 54 components were identified from Lonicerae Japonicae Flos extract, including 21 phenolic acids, 13 flavonoids, 12 iridoids, 4 triterpenoids and 4 other compounds. Therefore, DI-MS/MS~(ALL) is a powerful tool for comprehensive, rapid qualitative analysis of chemical profiles of traditional Chinese medicine and other chemical components of complex systems.
