Quality evaluation of Saposhnikoviae Radix based on QAMS combined with information entropy-response surface method.
10.19540/j.cnki.cjcmm.20201013.201
- Author:
Mei GUO
1
;
Qi AN
1
;
Ya-Jun SHEN
1
;
Dan-Hui ZHAO
2
;
Long GUO
1
;
Yu-Guang ZHENG
3
;
Dan ZHANG
1
Author Information
1. College of Pharmacy, Hebei University of Chinese Medicine Shijiazhuang 050200, China Traditional Chinese Medicine Processing Technology Innovation Center of Hebei Province, Hebei University of Chinese Medicine Shijiazhuang 050200, China.
2. College of Pharmacy, Hebei University of Chinese Medicine Shijiazhuang 050200, China.
3. College of Pharmacy, Hebei University of Chinese Medicine Shijiazhuang 050200, China Traditional Chinese Medicine Processing Technology Innovation Center of Hebei Province, Hebei University of Chinese Medicine Shijiazhuang 050200, China Hebei Chemical and Pharmaceutical College Shijiazhuang 050026, China.
- Publication Type:Journal Article
- Keywords:
Saposhnikoviae Radix;
information entropy;
quality evaluation;
quantitative analysis of multi-components by single marker(QAMS);
response surface method(RSM)
- MeSH:
Apiaceae;
Chromatography, High Pressure Liquid;
Drugs, Chinese Herbal;
Entropy;
Plant Roots
- From:
China Journal of Chinese Materia Medica
2021;46(10):2537-2546
- CountryChina
- Language:Chinese
-
Abstract:
This research was used with high performance liquid chromatography(HPLC), combined with information entropy-response surface method(RSM) to investigate the ethanol concentration, extraction time, liquid-to-material ratio. Taking the content of four chromogens as evaluation indexes, the weight coefficients of each index were given, and the comprehensive score was calculated to optimize the extraction process. Then, prim-O-glucosylcimifugin was used as the reference, the relative calibration factors(RCFs) of cimifugin, 4'-O-β-D-glucosyl-5-O-methylvisamminol and sec-O-glucosylhamaudo to prim-O-glucosylcimifugin were calculated respectively. The contents of four components in Saposhnikoviae Radix were determined by both external standard method(ESM)and quantitative analysis of multi-components by single marker(QAMS) method, and the results were compared. At last, combined with principal component analysis(PCA) and orthogonal partial least squares discriminant analysis(OPLS-DA) to evaluate the quality of the Saposhnikoviae Radix in different production areas. The optimal extraction process parameter of the Saposhnikoviae Radix was as follows: liquid-to-material ratio is 60∶1(mL·g~(-1)), extraction time is 35 min, and ethanol concentration is 70%. The repeatability of the RCFs was perfect, and the results calculated by the QAMS were consistent with the results from the ESM. The stoichiometric results indicate that there are obvious differences in the distribution of Saposhnikoviae Radix in different production areas, and cimifugin and prim-O-glucosylcimifugin are the characteristic compounds that cause this difference. In this study, the optimal extraction process is stable and feasible, and the method of QAMS is accurate and reliable. From the perspective of four chromogens, there are differences in the quality of the Saposhnikoviae Radix in different production areas. Therefore, the established extraction process combined with the method of QAMS can be used to evaluate the quality of Saposhnikoviae Radix and provide a scientific basis for the quality control of Saposhnikoviae Radix.
