Analysis of chemical constituents of Ginseng-Douchi compound fermentation products based on UPLC-Q-TOF-MS.
10.19540/j.cnki.cjcmm.20210223.301
- Author:
Jian-Feng SUN
1
;
Wen-Ting DONG
1
;
Li-Yan CHEN
1
;
Guo-Dong SUN
1
;
Jin-Hai HUO
1
;
Wei-Ming WANG
1
Author Information
1. Institute of Chinese Materia Medica,Heilongjiang Academy of Chinese Medical Sciences Harbin 150036,China.
- Publication Type:Journal Article
- Keywords:
Ginseng-Douchi;
UPLC-Q-TOF-MS;
component identification;
component transformation;
ferment
- MeSH:
Chromatography, High Pressure Liquid;
Drugs, Chinese Herbal;
Fermentation;
Panax;
Tandem Mass Spectrometry
- From:
China Journal of Chinese Materia Medica
2021;46(6):1417-1429
- CountryChina
- Language:Chinese
-
Abstract:
In this experiment, ultra high performance liquid chromatography-quadrupole time-of-flight mass spectrometry(UPLC-Q-TOF-MS) was used to analyze and identify chemical constituents of Ginseng-Douchi(GD) compound fermentation, and explore the conversion rules of ginsenosides and soybean isoflavones after compound fermentation. Waters Acquity UPLC BEH C_(18) column(2.1 mm×100 mm, 1.7 μm) was adopted, with 0.1% formic acid aqueous solution(A)-0.1% formic acid acetonitrile solution(B) as mobile phase for gradient elution; electrospray ion source(ESI) was used to collect data in positive and negative ion modes; according to the exact mass number, the secondary spectrum comparison of the database and the existing literature reports, Peakview 2.0/masterview 1.0 software was used to determine the common ion structure formula. Finally, a total of 133 chemical constituents were analyzed and identified from the GD. Ginseng saponins and isoflavone glycosides were significantly converted after fermentation. Among them, peak areas of prototype ginsenosides Rk_3, Rh_1, Rh_2, Rh_3, daidzin, glycitin and genistin decreased significantly; whereas peak areas of se-condary ginsenoside Rb_1, Rb_2, Rk_1, glycitein, genistein and daidzein increased significantly. In this experiment, liquid-mass spectrometry technique was used to investigate the conversion of active ingredients of GD compound fermented products after co-fermentation, so as to provide a scientific basis for elucidating pharmacodynamics material basis and quality control.
