Correlation study on non-volatile ingredients of Menthae Haplocalycis Herba formula granules and traditional herbal pieces by UPLC-MS/MS.
10.19540/j.cnki.cjcmm.20201221.301
- Author:
Ya-Qin ZHEN
1
;
Wei TIAN
1
;
Ya-Jing ZHI
2
;
Meng WANG
1
;
Zhi-Yuan LI
3
;
Li-Ying NIU
1
Author Information
1. Hebei University of Chinese Medicine Shijiazhuang 050091,China Hebei Traditional Chinese Medicine (TCM) Formula Granule Innovation Center Shijiazhuang 050091,China Hebei TCM Quality Evaluation & Standardization Engineering Research Center Shijiazhuang 050091,China.
2. Hebei University of Chinese Medicine Shijiazhuang 050091,China.
3. SCIEX China Shanghai 200335,China.
- Publication Type:Journal Article
- Keywords:
Menthae Haplocalycis Herba;
UPLC-MS/MS;
content determination;
correlation;
formula granules;
traditional herbal pieces
- MeSH:
Chromatography, High Pressure Liquid;
Chromatography, Liquid;
Correlation of Data;
Drugs, Chinese Herbal;
Tandem Mass Spectrometry
- From:
China Journal of Chinese Materia Medica
2021;46(5):1134-1140
- CountryChina
- Language:Chinese
-
Abstract:
To establish the method for determining non-volatile ingredients of neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, caffeic acid, isochlorogenic acid A, rosmarinic acid, ferulic acid, rutin, luteoloside, isoquercitrin, hesperidin, diosmin, diosmetin, luteolin, acacetin and linarin in Menthae Haplocalycis Herba formula granules and traditional herbal pieces by UPLC-MS/MS, and analyze the correlation of non-volatile ingredients in Menthae Haplocalycis Herba formula granules and traditional herbal pieces. Shim-pack GIST C_(18) column(2.1 mm×100 mm, 2 μm) was adopted with acetonitrile-0.1% formic acid aqueous solution as the mobile phase for gradient elution at the flow rate of 0.4 mL·min~(-1). The column temperature was set at 35 ℃. The quantitative analysis was performed using the electrospray ionization source and the multiple reaction monitoring. The linear relationship, resolution, repeatability and recovery of the 16 chemical components all met the requirements. The 16 non-volatile ingredients in traditional herbal pieces of Menthae Haplocalycis Herba could be tracked in formula granules. There were certain differences of the 16 chemical components among Menthae Haplocalycis Herba formula granules of different manufacturers and traditional herbal pieces of different producing areas. The UPLC-MS/MS method was simple, rapid and accurate, and could be used for the quality control of non-volatile ingredients in Menthae Haplocalycis Herba formula granules and traditional herbal pieces.
