Optimization of determination of astragaloside Ⅳ in Astragali Radix by continuous single-factor method.
10.19540/j.cnki.cjcmm.20200706.203
- Author:
Yao-Hua LIANG
1
;
Chun LI
1
;
Wei-Hong FENG
1
;
Xiao-Qian LIU
1
;
Liang-Mian CHEN
1
;
Jing-Jing ZHU
1
;
Li-Hua YAN
1
;
Chen-Xiao-Ning MENG
1
;
Zhong-Yuan GUO
2
;
Hui-Ming GAO
1
;
Zhi-Min WANG
3
Author Information
1. Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences Beijing 100700, China National Engineering Laboratory for Quality Control Technology of Chinese Herbal Medicines Beijing 100700, China.
2. Key Laboratory of SATCM for Process Analysis Technology of Chinese Herbal Medicines Beijing 100700, China.
3. Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences Beijing 100700, China National Engineering Laboratory for Quality Control Technology of Chinese Herbal Medicines Beijing 100700, China Key Laboratory of SATCM for Process Analysis Technology of Chinese Herbal Medicines Beijing 100700, China.
- Publication Type:Journal Article
- Keywords:
Astragali Radix;
astragaloside Ⅳ;
continuous single-factor method;
determination;
optimization
- MeSH:
Astragalus Plant;
Chromatography, High Pressure Liquid;
Drugs, Chinese Herbal;
Saponins;
Triterpenes/analysis*
- From:
China Journal of Chinese Materia Medica
2021;46(2):391-397
- CountryChina
- Language:Chinese
-
Abstract:
This paper aims to solve the problems of complicated-unstable test solution preparation process and insufficient extraction of the active ingredient astragaloside Ⅳ in the legal method for the determination of astragaloside Ⅳ in Astragali Radix. The continuous single-factor analysis of seven main factors affecting the content of astragaloside Ⅳ was carried out by HPLC-ELSD, and then the pre-paration method of test solution was optimized. This optimized method exhibited excellent performance in precision, repeatability and stability. The average recovery rate of astragaloside Ⅳ was 99.65% with RSD 2.2%. Astragaloside Ⅳ showed a good linearity between the logarithm of peak area and the logarithm of injection quantity in the range of 0.46-9.1 μg(r=0.999 6). The contents of astragaloside Ⅳ in 29 batches of Astragali Radix were determined by the new and the legal methods. The results showed that the average content of astragaloside Ⅳ in these Astragali Radix samples determined by the former method was 1.458 times than that of the latter one, indicating the new method was simple, reliable and more adequate to extract target compound. According to the results, it is suggested to improve the content standard of astragaloside Ⅳ in Astragali Radix in the new edition of Chinese Pharmacopeia.
