Pharmacokinetic study on five components in phthalide target areas of Chaxiong and its β-CD inclusion compounds based on UPLC-MS/MS.
10.19540/j.cnki.cjcmm.20201012.201
- Author:
Ying-Huai ZHONG
1
;
Jian-Fang FENG
1
;
Ming-Yan XIA
2
;
Xin-Hua WEI
3
;
Qiu-Yan WU
2
;
Dong-Xun LI
2
;
Xue-Mei LIU
4
;
Guo-Song ZHANG
5
Author Information
1. Jiangxi University of Traditional Chinese Medicine Nanchang 330006,China College of Pharmacy,Guangxi University of Traditional Chinese Medicine Nanning 530200,China.
2. Jiangxi University of Traditional Chinese Medicine Nanchang 330006,China.
3. National Pharmaceutical Engineering Center For Solid Preparation in Chinese Herbal Medicine Nanchang 330006,China.
4. College of Pharmacy,Guangxi University of Traditional Chinese Medicine Nanning 530200,China.
5. Jiangxi University of Traditional Chinese Medicine Nanchang 330006,China National Pharmaceutical Engineering Center For Solid Preparation in Chinese Herbal Medicine Nanchang 330006,China.
- Publication Type:Journal Article
- Keywords:
Lgusticum sinense Oliv.cv.Chaxiong;
UPLC-MS/MS;
Z-ligustilide;
new osthol lactone;
pharmacokinetic;
senkyunolide A;
target area
- MeSH:
Animals;
Benzofurans;
Chromatography, High Pressure Liquid;
Chromatography, Liquid;
Rats;
Rats, Sprague-Dawley;
Reproducibility of Results;
Tandem Mass Spectrometry
- From:
China Journal of Chinese Materia Medica
2021;46(4):972-980
- CountryChina
- Language:Chinese
-
Abstract:
This study aims to establish a method for the determination of the concentration of five main components of phthalide target areas of Chaxiong(CPTA) and its inclusion of β-CD in the plasma of rats, and determine the pharmacokinetic parameters, absolute bioavailability and relative bioavailability of CPTA/β-CD inclusion compound in vivo. The plasma concentrations of senkyunolide A, N-butylphthalide, new osthol lactone, Z-ligustilide and butenyl phthalide were determined with UPLC-MS/MS. The content determination was conducted at the chromatographic conditions as follows: Shim-pack GIST C_(18)-AQ HP column(2.1 mm×100 mm, 3 μm), mobile phase of 0.1% formic acid solution(A)-acetonitrile(B), gradient elution, flow rate of 0.3 mL·min~(-1), column temperature of 35 ℃ and injection volume of 2 μL. The mass spectra were obtained with electrospray ion source(ESI), positive ion mode and multi reaction monitoring. CPTA/β-CD inclusion compound was prepared by grinding method, DAS 2.0 software was used to model the data, and the absolute bioavailability of CPTA and relative bioavailability of inclusion compound were calculated. Finally, the methods for the determination of five components of senkyunolide A, N-butylphthalide, new osthol lactone, Z-ligustilide and butenyl phthalide in CPTA, were successfully established. The linear relationship among the five components was good within their respective ranges, r>0.99. The absolute bioavailability of the five components in rats was 22.30%, 16.32%, 21.90%, 10.16% and 12.43%, respectively. After CPTA/β-CD inclusion was prepared, the relative bioavailability of the five components was 138.69%, 198.39%, 218.01%, 224.54% and 363.55%, respectively, significantly improved. This method is rapid, accurate and sensitive, so it is suitable for the pharmacokinetic study of extracts in traditional Chinese medicine and their preparations.
