Quality evaluation of fried Glycyrrhizae Radix et Rhizoma pieces by HPLC fingerprint and multicomponent quantitative analysis.
10.19540/j.cnki.cjcmm.20201022.306
- Author:
Shu-Hui CAI
1
;
Hua-Cong ZHAO
1
;
Meng JIA
1
;
Xiao-Li ZHAO
1
;
Yu-Mei CHI
2
;
Wen ZHANG
1
;
Hong-Lan WANG
1
;
Liu-Qing DI
1
Author Information
1. School of Pharmacy, Nanjing University of Chinese Medicine Nanjing 210023, China Jiangsu Engineering Research Center for Efficient Delivery System of Traditional Chinese Medicine Nanjing 210023, China.
2. School of Pharmacy, Nanjing University of Chinese Medicine Nanjing 210023, China.
- Publication Type:Journal Article
- Keywords:
fingerprint;
fried Glycyrrhizae Radix et Rhizoma pieces;
multicomponent quantitative analysis;
pattern recognition;
quality evaluation
- MeSH:
Chromatography, High Pressure Liquid;
Drugs, Chinese Herbal;
Plant Extracts;
Quality Control
- From:
China Journal of Chinese Materia Medica
2021;46(1):118-124
- CountryChina
- Language:Chinese
-
Abstract:
To establish the HPLC fingerprint and multi-component determination method of fried Glycyrrhizae Radix et Rhizoma pieces. HPLC analysis was performed on Thermo Acclaim ~(TM)120 C_(18) column(4.6 mm×250 mm, 5 μm). Acetonitrile-0.1% phosphoric acid aqueous solution was taken as the mobile phase for gradient elution. The flow rate was 1 mL·min~(-1),the column temperature was maintained at 30 ℃, and the detection wavelength was 237 nm and 360 nm. The similarity of 15 batches of fried Glycyrrhizae Radix et Rhizoma pieces was higher than 0.849, and 17 common peaks were identified. Liquiritin, isoliquiritin apioside, isoliquiritin, liquiritigenin, isoliquiritigenin and glycyrrhizic acid were identified; among them, the mass fractions of Liquiritin, isoliquiritin apioside, isoliquiritin, liquiritigenin, glycyrrhizic acid were were 0.519%-3.058%, 0.227%-0.389%, 0.070%-0.439%, 0.038%-0.173%, 1.381%-4.252%, respectively. According to the cluster analysis, the 15 batches of decoction pieces were classified into three categories; principal component analysis screened out four principal components, with the cumulative variance contribution rate of 86.630%, indicating that the principal components contained most information of original data. Partial least squares discriminant ana-lysis marked 6 differential components in the decoction pieces. The established fingerprint and multicomponent determination are stable and reliable, and can provide a reference for the quality control of Radix Glycyrrhizae Radix et Rhizomae and fried Glycyrrhizae Radix et Rhizoma pieces.
