Effect of the regulator of G-protein signaling 2 on the proliferation and invasion of oral squamous cell carcinoma cells and its molecular mechanism.

Cheng-Zhong LIN; Zhe-Qi LIU; Wen-Kai ZHOU; Tong JI; Wei CAO

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Effect of the regulator of G-protein signaling 2 on the proliferation and invasion of oral squamous cell carcinoma cells and its molecular mechanism.

Author: Cheng-Zhong LIN ¹ ; Zhe-Qi LIU ² ; Wen-Kai ZHOU ² ; Tong JI ² ; Wei CAO ²
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1. The 2nd Dental Center, Ninth People,s Hospital, Shanghai Jiao Tong University School of Medicine; National Clinical Research Center for Oral Disease; Shanghai Key Laboratory of Stomatology & Shanghai Research Institute of Stomatology, Shanghai 200011, China.
2. Dept. of Oral and Maxillofacial-Head and Neck Oncology, Ninth People,s Hospital, Shanghai Jiao Tong University, School of Medicine; National Clinical Research Center for Oral Disease; Shanghai Key Laboratory of Stomatology & Shanghai Research Institute of Stomatology, Shanghai 200011, China.
Publication Type:Journal Article
Keywords: four and a half LIM domains protein 1; invasion; oral squamous cell carcinoma; proliferation; regulator of G-protein signaling 2; ubiquitination
MeSH: Carcinoma, Squamous Cell; Cell Line, Tumor; Cell Movement; Cell Proliferation; GTP-Binding Proteins; Head and Neck Neoplasms; Humans; Intracellular Signaling Peptides and Proteins; LIM Domain Proteins; Mouth Neoplasms; Muscle Proteins; Squamous Cell Carcinoma of Head and Neck
From: West China Journal of Stomatology 2021;39(3):320-327
CountryChina
Language:English
Abstract: OBJECTIVES:This study aims to investigate the effect of the regulator of G-protein signaling 2 (RGS2) on the proliferation and invasion of oral squamous cell carcinoma (OSCC) cells and its potential molecular mechanism. Metho⁃ds The expression status and clinical significance of RGS2 in head and neck squamous cell carcinomas and matched adjacent normal tissues were evaluated using TCGA database. Three OSCC cell lines (i.e., SCC-9, Cal27, and Fadu) were overexpressed with RGS2, and the effect of RGS2 on cell proliferation and invasion was determined using the Transwell, clone formation, and cell counting kit (CCK)-8 assays. Moreover, the yeast two-hybrid scree-ning and co-immunoprecipitation (Co-IP) assays were conducted to detect the correlation of RGS2, four and a half LIM domains protein 1 (FHL1), and damage DNA-binding protein 1 (DDB1).
RESULTS:The expression level of RGS2 in OSCC was significantly lower than that in matched adjacent normal tissues (
CONCLUSIONS:RGS2 plays an important role in the inhibition of OSCC proliferation and invasion. The structure stability of RGS2 is competitively regulated by FHL1 and DDB1.