Study on the mechanism of resistance to cypermethrin in Culex pipiens pallens using proteomics
10.16250/j.32.1374.2020347
- VernacularTitle:利用蛋白组学解析淡色库蚊对氯氰菊酯的抗性机制
- Author:
Fu-Yan LIU
1
;
Qian ZHANG
2
;
Xiu-Xia GUO
3
;
Xiao SONG
3
;
Chong-Xing ZHANG
3
Author Information
1. Shandong Institute of Parasitic Diseases, Shandong First Medical University & Shandong Academy of Medical Sciences, Jining 272000, China; Clinical Laboratory, Affiliated Hosptial of Jining Medical University, China
2. Jining Municipal Hospital of Traditional Chinese Medicine, Shandong Province, China
3. Shandong Institute of Parasitic Diseases, Shandong First Medical University & Shandong Academy of Medical Sciences, Jining 272000, China
- Publication Type:Journal Article
- Keywords:
Culex pipiens pallens;
Cypermethrin;
Insecticide resistance;
Proteomics;
Isobaric tags for relative and absolute quantification (iTRAQ);
Liquid chromatography with tandem mass spectrometry
- From:
Chinese Journal of Schistosomiasis Control
2021;33(2):189-194
- CountryChina
- Language:Chinese
-
Abstract:
Objective To compare the differentially expressed proteins between cypermethrin-resistant and -sensitive Culex pipiens pallens, so as to unravel the mechanism underlying the resistance to cypermethrin in Cx. p. pallens. Methods A quantitative proteomic analysis was performed among cypermethrin-sensitive and -resistant isolates of Cx. p. pallens using isobaric tags for relative and absolute quantification (iTRAQ) labeling coupled with liquid chromatography with tandem mass spectrometry (LC-MS/MS). Results A total of 164 differentially expressed proteins were identified between cypermethrin-sensitive and -resistant isolates of Cx. p. pallens, including 54 up-regulated proteins and 110 down-regulated proteins. A large number of cuticular proteins, larval cuticular proteins, pupal cuticular proteins and cuticular structural constituent proteins, which are associated with cytoskeletal structure and components, were differentially expressed between cypermethrin-sensitive and -resistant isolates of Cx. p. pallens. Thirteen proteins, which were involved in energy production and conversion, translation, ribosomal structure and biogenesis, lipid transport and metabolism, post-translational modification, protein turnover, chaperones, cytoskeleton and intracellular transportation, were validated to be differentially expressed between cypermethrin-sensitive and -resistant isolates of Cx. p. pallens, which may serve as potential markers of cypermethrin resistance. Conclusion Multiple insecticide resistance mechanisms contribute to the resistance to cypermethrin in Cx. p. pallens, including cuticular resistance and metabolic resistance, and the cuticular protein genes and cytochrome P450 enzymes may play an important role in the resistance of Cx. p. pallens to cypermethrin.
