Screening of Main Effective Components of Compound Agrimonia pilosula Enteritis Capsules and Targets of Enteritis
- VernacularTitle:复方仙鹤草肠炎胶囊的主要有效成分及其治疗肠炎靶点的筛选
- Author:
Jun LI
1
;
Yingxian WANG
1
;
Jiasheng LI
1
;
Yi ZHANG
1
;
Ruirui WANG
1
Author Information
1. College of Chinese Materia Medica，Yunnan University of Chinese Medicine，Kunming 650500，China
- Publication Type:Journal Article
- Keywords:
Compound Agrimonia pilosula enteritis capsules;
Network pharmacology;
Chemical components;
Enteritis;
Targets
- From:
China Pharmacy
2021;32(8):927-932
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE：To screen the main effective components of Compound Agrimonia pilosula enteritis capsules and targets of enteritis. METHODS ：UHPLC-MS/MS，MWDB database and relevant literature analysis were used to identify main chemical components in methanol extract of Compound A. pilosula enteritis capsules. TCMSP ，PubChem and UniProt database were adopted to predict and screen the active ingredients and their potential targets. GeneCards and OMIM database were used to predict and screen enteritis related targets ；common targets were screened by R language 4.0.2. The chemical components corresponding to the common targets were matched with the chemical components in the methanol extract of the preparation to obtain the main effective components of the preparation. With the help of STRING database and Cytoscape 3.7.1 software，the protein-protein interaction network was constructed ，and the key targets of the preparation were screened by degree. RESULTS ： A total of 48 compounds were identified ，including 13 phenolic acids ，10 alkaloids，8 flavonoids，6 terpenoids，6 other compounds，3 lipids，1 tannin and 1 organic acid. Compared with the network pharmacology data ，apigenin，luteolin，quercetin （3，7-di-O-methylquercetin，Quercetin-3-O-β-D-galactoside，Quercetin-7-O-glucoside，Quercetin-3-O-β-D-glucoside），palmatine， protocatechuic acid- 4-glucoside and 3，4-dimethoxycinnamic acid were the main effective components. The key targets for the treatment of enteritis included RELA ，APP，CCND1，EGFR，INS，ESR1，IL6，NCOA1，CASP8，FOS. CONCLUSIONS ：A total of 9 main effective components （including apigenin ，luteolin，quercetin，etc.）and 10 key targets for enteritis （including RELA，APP，CCND1，etc.）of Compound A. pilosula enteritis capsules are found.
