Effect of over-expression of vasohibin-1 on malignant biological behavior of human colorectal cancer cells
DOI: 10.3872/j.issn.1007-385x.2021.02.004
- VernacularTitle:过表达血管生成抑制蛋白1 对人结直肠癌细胞恶性生物学行为的影响
- Author:
GAO Weifeng1
1
;
ZHANG Chunze1, 2
1
,
2
;
ZHANG Qinghuai1
1
;
SUN Jing3
3
;
SU Yanjun4
4
;
ZHANG Weihua1
1
Author Information
1. Department of Colorectal Surgery, Tianjin Union Medical Center, Tianjin 300121, China;
2. State Key Laboratory of Medicinal Chemical Biology, Nankai University, Tianjin 300071, China;
3. Department of Laboratory, Tianjin Union Medical Center, Tianjin 300121, China
4. Department of Lung Cancer, Tianjin Medical University Cancer Institute and Hospital, Tianjin 300060, China
- Publication Type:Journal Article
- Keywords:
colorectal cancer;
vasohibin-1 (VASH1);
microangiogenesis;
proliferation;
metastasis
- From:
Chinese Journal of Cancer Biotherapy
2021;28(2):121-127
- CountryChina
- Language:Chinese
-
Abstract:
[Abstract] Objective: To investigate the effects of over-expressing VASH1 on the malignant biological behaviors of human colorectal
cancer cells. Methods: Lentivirus was packaged and transfected into human colorectal cancer cells SW680 and SW620 to construct an
over-expressed VASH1 cell line, the untransfected cells were used as control. qPCR experiment and WB experiment were used to detect
the over-expression effect of VASH1. The effects of VASH1 over-expressed on microangiogenesis, proliferation, colony formation and
migration of colorectal cancer cells were detected respectively by tubule formation, CCK-8 assay, soft agar assay, Transwell assay and
Wound healing assay in vitro. In addition, tumor growth and lung metastasis were detected in NOD-SCID mice subcutaneously injected
with VASH1-overexpressing SW620 cells. Results: Successfully constructed SW480 and SW620 cells over-expressing VASH1.
Compared with the control group, the abilities of microangiogenesis, proliferation, colony forming and migration were significantly
reduced in colorectal cancer cells over-expressing VASH1 (P<0.05) in vitro. The abilities of subcutaneous tumor growth and lung
metastasis of colorectal cancer cells over-expressing VASH1 were also significantly reduced (P<0.05) in vivo. Conclusion: Over-expression
of VASH1 can suppress the malignant biological behaviors of human colorectal cancer cells.
- Full text:zgzlswzlzz-2021-2-121.pdf
