Analysis of Chemical Constituents in Bushao Tiaozhi Capsules by UPLC-Q-TOF-MS
10.13422/j.cnki.syfjx.20201613
- VernacularTitle:基于UPLC-Q-TOF-MS的布芍调脂胶囊化学成分分析
- Author:
Guan-lin XIAO
1
;
Jie-yi JIANG
1
;
Ai-li XU
1
;
Yang-xue LI
1
;
Xiao-li BI
1
Author Information
1. （Guangdong Province Engineering Technology Research Institute of Traditional Chinese Medicine（TCM），Guangdong Provincial Key Laboratory of Research and Development in TCM， Guangzhou 510095，China）
- Publication Type:Research Article
- Keywords:
Bushao Tiaozhi capsules;
ultra-performance liquid chromatography coupled to quadrupole time-of-flight mass spectrometry;
flavonoids;
phenolics;
terpenes
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2020;26(14):190-199
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To analyze the main chemical constituents of traditional Chinese medicine compound Bushao Tiaozhi capsules by using UPLC-Q-TOF-MS technology. Method:The separation was eluted with Waters CORTECS UPLC C18 column（2.1 mm×150 mm，1.6 μm） in a gradient mode， with methanol-0.1% formic acid as the mobile phase. The column temperature was 40 ℃，the flow rate was 0.24 mL·min-1，and the injection volume was 1 μL. The mass spectrometry condition was X500R QTOF mass spectrometry，the positive and negative electrospray ionization （ESI） was adopted for determine the chromatographic effluent，and the main chromatographic peaks were assigned and distinguished by Q-TOF. Result:A total of 53 chemical constituents were identified by reference confirmation，literature comparison，and high mass spectrometry data analysis. The chemical constituent cluster was composed of 21 flavonoids，10 phenolics，5 monoterpene glycosides，7 diterpene lactones and 10 sesquiterpenes. Furthermore，all of the constituents were surveyed and classified according to their medicinal materials derivation. Among them，the 5 flavonoids components（mangiferin，isoquercitrin，typhaneoside，isorhamnetin 3-O-neohesperidoside，tiliroside）were identified in Microctis Folium for the first time. Conclusion:This study shows that UPLC-Q-TOF-MS technology provides a simple，rapid，and accurate method for the identification of chemical constituents in Bushao Tiaozhi capsules. The identified chemical components mostly cover the main constituents of each medicinal material in the formula，so as to provide a new technological method and theoretical foundation for further defining the pharmacological basis and mechanism of action and optimizing the quality control of Bushao Tiaozhi capsules.
