Effect of Mixed Inoculation of Arbuscular Mycorrhizal on Growth and Chemical Composition of Aucklandia lappa Seedlings
10.13422/j.cnki.syfjx.20201614
- VernacularTitle:混合接种丛枝菌根真菌对木香幼苗生长及化学成分的影响
- Author:
Wen-chao GU
1
;
Wen-wu YANG
2
;
Jie ZHANG
2
;
Min YANG
1
;
Bo DING
2
;
Dong-qin GUO
2
;
Xing-jiao PAN
1
;
Yan-bin XUE
2
;
Nong ZHOU
2
Author Information
1. College of Pharmacy and Chemistry，Dali University，Dali 671000，China
2. College of Food and Biological Engineering，The Chongqing Engineering Laboratory for Green Cultivation and Deep Processing of the Three Gorges Reservoir Area's Medicinal Herbs，Chongqing Three Gorges University，Chongqing 404120，China
- Publication Type:Research Article
- Keywords:
Aucklandia lappa;
arbuscular mycorrhizal fungi;
growth characteristics;
physiological characteristics;
biomass;
costunolide;
dehydrocostus lactone
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2020;26(14):173-181
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the synergistic effect of arbuscular mycorrhizal（AM） fungi mixed inoculation on the growth，physiological and biochemical characteristics，root biomass and terpenoid component accumulation of Aucklandia lappa seedlings，so as to provide a reference for the combination and application of the dominant complementary effect mycorrhizal fungi. Method:The effect of different AM fungi combined with inoculation on the root mycorrhizal infection rate，plant growth，physiological and biochemical characteristics，root biomass，costunolide and dehydrocostus lactone of A. lappa seedlings were determined by pot inoculation at room temperature. Result:It was found that AM fungi could form good mycorrhizal symbiosis with the roots of A. lappa.The formation of mycorrhizal symbiosis system could increase the chlorophyll content of A. lappa leaves，increase the activities of catalase（CAT），peroxidase （POD），superoxide dismutase （SOD），reduce the content of malondialdehyde（MDA），and promote photosynthesis of A. lappa. Compared with CK group，AM fungus treatment could significantly promote the accumulation of costunolide and dehydrocostus lactone，and the accumulation of its metabolites，costunolide and dehydrocostus lactone，into roots during the symbiotic cultivation of A. lappa seedlings，indirectly improving the quality of medicines and yield of alantolactone. Conclusion:Inoculation of AM fungi can improve the root mycorrhizal viability，increase the absorption of nutrients and promote the growth of woody incense.The mixed inoculation treatment of S2，S4 and S5 had the best mycorrhizal effect in artificial cultivation，and the growth and medicinal quality of A. lappa were the best，which provided technical support for the application and popularization of A. lappa mycorrhizal biotechnology.
