Analysis of Effect of Realgar with Different Compatibility Proportions on Transitional Constituents of Indigo Naturalis in Rat Serum Based on UPLC-Q-TOF-MS/MS
10.13422/j.cnki.syfjx.20200648
- VernacularTitle:基于UPLC-Q-TOF-MS/MS技术分析不同配伍比例雄黄对青黛入血成分的影响
- Author:
Min ZHANG
1
;
Qing-qing WU
1
;
Chen-hui LI
1
;
Ling-ling XU
1
;
Yuan-yuan ZHOU
1
;
Peng HUANG
1
;
He-ping HUANG
1
;
Juan YU
1
;
Dian-lei WANG
1
Author Information
1. Anhui Province Key Laboratory of Chinese Medicinal Formula, School of Pharmacy, Anhui University of Chinese Medicine, Hefei 230012, China
- Publication Type:Research Article
- Keywords:
Indigo Naturalis;
Realgar;
Qinghuangsan;
serum pharmacochemistry;
transitional constituents;
alkaloids;
pharmacodynamic material basis
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2020;26(13):79-86
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of different dose of Realgar compatible with Indigo Naturalis on the transitional constituents of Indigo Naturalis in rat serum based on the compatibility of Qinghuangsan.Method:Indigo Naturalis test solution, the drug-containing serum of three different proportions of Qinghuangsan (10 g of Indigo Naturalis compatible with 52.5, 105, 210 mg of Realgar for group A, B and C, respectively) and blank serum were detected by UPLC-Q-TOF-MS/MS, in combination with the chemical components identified in Indigo Naturalis test solution, the differences of transitional constituents of Indigo Naturalis in rat serum from the group A, B and C were analyzed. HL-60 cells (human leukemia cells) were treated with the three groups of Qinghuangsan drug-containing serum and the effect of drug-containing serum on the activity of HL-60 cells was detected by cell counting kit-8 (CCK-8) assay.Result:A total of 19, 22, 25 of transitional constituents were detected in Qinghuangsan drug-containing serum from group A, B and C, respectively. The three groups of drug-containing serum all contained 5 prototype components from Indigo Naturalis test solution, including tryptanthrin, indigo, indirubin, 2-aminobenzoic acid and N-phenyl-2-naphthylamine, respectively. The results of CCK-8 assay showed that Qinghuangsan drug-containing serum of group C had the strongest inhibitory effect on HL-60 cells.Conclusion:After fixed Indigo Naturalis dose, with the increase of Realgar dose, the transitional constituents in rat serum increase and the inhibitory effect on HL-60 cells also gradually enhances, which indicates that Realgar may promote the absorption of active components in Indigo Naturali in vivo, thus enhance the efficacy, further explains the compatibility law and pharmacodynamic material basis of different proportions of Realgar and Indigo Naturalis.
