Effect of Modified Buwangsan on Expression of Related Molecules in NLRP3 Inflammatory Signaling Pathway in Hippocampus of AD Rats
10.13422/j.cnki.syfjx.20200239
- VernacularTitle:加味不忘散对AD模型大鼠海马区NLRP3炎症通路中相关因子表达的影响
- Author:
Ling-ling HE
1
;
Xiao-qiong LI
1
;
Xiao-lei LIU
1
;
Miao-miao HOU
1
;
Xin-yi LI
1
Author Information
1. Shanxi Dayi Hospital Affiliated to Shanxi Medical University, Taiyuan 030000, China
- Publication Type:Research Article
- Keywords:
Alzheimer's disease;
modified Buwangsan;
NOD-like receptor 3 (NLRP3) inflammasome;
cysteine containing aspartate specific proteases-1(Caspase-1);
interleukin-1 beta (IL-1β)
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2020;26(4):35-41
- CountryChina
- Language:Chinese
-
Abstract:
Objective::To investigate the effects of modified Buwangsan on the learning and memory ability of Alzheimer's disease (AD) model rats and the expression of NOD-like receptor 3 (NLRP3), cysteine-containing aspartate-specific proteases 1 (Caspase-1) and interleukin-1 beta (IL-1β) in NLRP3 inflammatory pathway in hippocampus of AD model rats, and exploring the underlying mechanism of modified Buwangsan. Method::The 52 eligible rats were randomly divided into sham control group, AD model group, low-dose modified Buwangsan group (1.5 g·kg-1) and high-dose modified Buwangsan group (3 g·kg-1). AD mouse model was established by bilateral hippocampus injection of Aβ1-425 μL (2 g·L-1). The rats in low-dose and high-dose modified Buwangsan group received low and high dose modified Buwangsan respectively within the next 4 weeks, once daily. The learning and memory ability was tested by Morris water maze. The expression of NLRP3, Caspase-1 and IL-1β mRNA was tested by quantitative PCR(Real-time PCR) and Western blot. Result::As compared with the sham group, the learning and memory ability of the rats were significantly impaired (P<0.05). Compared with AD model group, the learning and memory ability and the expression levels of NLRP3, Caspase-1, and IL-1β mRNA and protein were all no statistical differences in low-dose modified Buwangsan group, while the learning and memory ability of the rats were significantly improved and the expression of NLRP3, Caspase-1 and IL-1β mRNA in hippocampus of rats was significantly decreased in high-dose modified Buwangsan group (P<0.05). Conclusion::High-dose modified Buwangsan could attenuate neuroinflammation in the hippocampus of AD mouse model via inhibiting the expression of NLRP3, Caspase-1 and IL-1β, which may be the mechanisms of modified Buwangsan could be used to ameliorate the learning and memory ability of AD mouse model.
