Analysis on Chemical Characteristics of Lycii Fructus in Qinghai Province Based on 1H-NMR Plant Metabolomics
10.13422/j.cnki.syfjx.20201159
- VernacularTitle:基于1H-NMR植物代谢组学技术分析青海产区枸杞子的化学特征
- Author:
Guang-yue ZHAO
1
;
Yu-hai WEI
1
;
Shan-shan SU
1
;
Hong-ling LA
1
;
Pei-ying YAN
2
;
Cai-ming LI
2
;
Zhen-yu LI
3
Author Information
1. Key Laboratory of Food Safety Research in Qinghai Province,Technical Center of Xining Customs,Xining 810001,China
2. School of Life Sciences,Qinghai Normal University,Xining 810001,China
3. Modern Research Center for Traditional Chinese Medicine,Shanxi University,Taiyuan 030006,China
- Publication Type:Research Article
- Keywords:
Lycii Fructus;
proton nuclear magnetic resonance (1H-NMR);
chemical difference;
metabolomics;
similarity;
polysaccharides;
Pearson correlation
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2020;26(17):95-103
- CountryChina
- Language:Chinese
-
Abstract:
Objective:The chemical differences of Lycii Fructus samples from Qinghai, Ningxia, Gansu, Xinjiang and Inner Mongolia provinces were compared based on proton nuclear magnetic resonance (1H-NMR) plant metabolomics. Method:A total of 97 Lycii Fructus samples from five provinces were collected, including 61 samples in Qinghai, and extracted by 50% methanol for detecting. 1H-NMR spectra were obtained and compared by multivariate statistical analysis for investigating the chemical differences of samples from Qinghai and other production areas. And the content of Lycii Fructus polysaccharides in all samples was determined with the wavelength of 490 nm (calculated by anhydrous glucose). Result:A total of 32 chemical components were detected in the Lycii Fructus extract by 1H-NMR. The multivariate statistical analysis revealed that there was no significant difference among the samples from five provinces. The difference between Lycii Fructus from Qinghai and Ningxia, as well as the samples among the six regions of Qinghai province were relatively small. The similarity values of the majority of samples were >0.85. Univariate analysis showed that no significant difference was observed for the most metabolites in Lycii Fructus collected from five provinces, except for sucrose, glucose, proline and so on. There was no significant difference in the content of Lycii Fructus polysaccharides between Qinghai and other provinces. And the correlation coefficient between the content of Lycii Fructus polysaccharides and the small molecular compounds identified by 1H-NMR was -0.2-0.4. Conclusion:In this study, chemical characteristics of Lycii Fructus in Qinghai province are analyzed from the holistic view by 1H-NMR plant metabolomics, in combination of polysaccharide determination, and the results show that there is no significant difference between samples from Qinghai and other four provinces. The quality evaluation method based on 1H-NMR established in this study can provide scientific basis for improving quality control level and selecting planting areas of Lycii Fructus.
