Study on Effect and Mechanism of Huayu Jiedu Recipe on Apoptosis of Gastric Cancer Cells
10.13422/j.cnki.syfjx.20201927
- VernacularTitle:化瘀解毒方对胃癌细胞凋亡的作用及机制
- Author:
Zheng WEI
1
;
Ai-hua ZHANG
1
;
Ya-feng LI
1
;
Rui-feng LIANG
1
;
Wei-feng CUI
1
;
Yan-chun WANG
2
;
Jun-ping ZHANG
1
Author Information
1. Henan Academy of Chinese Medicine，Zhengzhou 450004，China
2. Henan Provincial People's Hospital，Zhengzhou 450000，China
- Publication Type:Research Article
- Keywords:
Huayu Jiedu recipe;
gastric cancer;
apoptosis;
mechanism
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2020;26(21):62-67
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe the effect of Huayu Jiedu recipe （HYJDR） on apoptosis of gastric cancer cells， explore the mechanism of HYJDR on apoptosis of gastric cancer cells and provide experimental basis for clinical application of HYJDR. Method:Thiazolyl blue tetrazolium bromide （MTT） method was used to detect the effect of HYJDR （5，10，15，20，25，30 g·L-1） on the activity of SGC-7901 cells and the median inhibition concentration （IC50） of HYJDR on SGC-7901 cells. Propidium iodide （PI） and Hoechst double fluorescence staining were used to detect the effect of HYJDR on the apoptosis of gastric cancer SGC-7901 cells. Western blot was used to detect the effect of HYJDR on the expression of apoptosis related proteins in gastric cancer cells. Real-time polymerase chain reaction （Real-time PCR） was used to detect the effect of HYJDR on the expression of apoptosis related protein mRNA in gastric cancer cells. Result:As compared with blank group， HYJDR （>10 g·L-1） can significantly inhibit the proliferation of gastric cancer cells. With the increase of the concentration of HYJDR， the cell viability decreased significantly obviously in a concentration-dependent manner（P<0.05）. HYJDR can significantly promote the apoptosis of gastric cancer cells. With the increase of the concentration of HYJDR， the apoptosis of gastric cancer cells gradually increased. As compared with blank group， HYJDR can obviously inhibit the expression of apoptosis-related B -cell lymphoma-2 （Bcl-2） （P<0.05）， and increase the expression of apoptosis-promoting proteins such as Bcl-2 antagonist of cell death （Bad） and Bcl-2-associated X （Bax）（P<0.05）. Real-time PCR results showed that as compared with blank group， HYJDR（5，10 g·L-1） could effectively inhibit the expression of Bcl-2 protein mRNA in gastric cancer cells（P<0.05）， and all HYJDR groups could promote the expression level of Bad mRNA（P<0.05）. Conclusions:HYJDR can obviously promote the apoptosis of SGC-7901， and its effect is probably achieved by increasing the expression of Bad mRNA and inhibiting the expression of Bcl-2 protein.
