Effects of Active Components of Naoxintong Capsule on the Proliferation of HUVEC and Its JAK/STAT Signal Pathway，Vasoactive Substances ，Adhesion Molecules and Inflammatory Factors
- VernacularTitle:脑心通胶囊药效成分对HUVEC增殖及其JAK/STAT信号通路、血管活性物质、黏附分子、炎症因子的影响
- Author:
Weixia LI
1
;
Xiaoyan WANG
1
;
Wenhui JIA
2
;
Mingliang ZHANG
3
;
Jinfa TANG
1
;
Xuelin LI
3
Author Information
1. Dept. of Pharmacy，the First Affiliated Hospital of Henan University of TCM/Henan Province Engineering Laboratory for Clinical Evaluation Technology of Chinese Medicine/Engineering Research Center for Modernization of Clinical Application of TCM Decoction Pieces in Henan Province，Zhengzhou 450008，China;College of Pharmacy/ Provincial and Ministerial Co-construction Collaborative Innovation Center for Prevention and Treatment of Δ 基金项目 ：国家自然科学基金资助项目 （No.82004082，No. Respiratory Diseases with Traditional Chinese U1504827）
2. College of Pharmacy/ Provincial and Ministerial Co-construction Collaborative Innovation Center for Prevention and Treatment of Δ 基金项目 ：国家自然科学基金资助项目 （No.82004082，No. Respiratory Diseases with Traditional Chinese U1504827）
3. Dept. of Pharmacy，the First Affiliated Hospital of Henan University of TCM/Henan Province Engineering Laboratory for Clinical Evaluation Technology of Chinese Medicine/Engineering Research Center for Modernization of Clinical Application of TCM Decoction Pieces in Henan Province，Zhengzhou 450008，China
- Publication Type:Journal Article
- Keywords:
Naoxintong capsule;
Human umbilical vein endothelial cells;
Vasoactive substances;
Adhesion molecule
- From:
China Pharmacy
2021;32(3):301-308
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE：To study the effects of the main active components of Naoxintong capsule （NXTC）on the proliferation of human umbilical vein endothelial cell（HUVEC） and its key protein JAK/STAT signal pathway ， vasoactive substances ，adhesion molecules and inflammatory factors so as to clarify the m echanism of NXTC for promoting blood circulation and removing blood stasis. METHODS ：The effects of different concentration of 12 active components [caffeic acid（1.56-200 μmol/L），ferulic acid （1.56-200 μmol/L），senkyunolide H （3.125-200 μmol/L），n-butylidenephthalide（3.125-200 μmol/L），ligustilide（1.56-200 μmol/L），cryptotanshinone（0.625-80 μmol/L），tanshinol sodium （1.56-200 μmol/L），paeoniflorin （1.56-200 μmol/L），formononetin（1.56-200 μmol/L），salvianolic acid B （1.56-200 μmol/L），catechin（1.56-200 μmol/L）and astragaloside Ⅳ（1.56-200 μmol/L）] on the proliferation of HUVECs were evaluated by CCK- 8 assay. The effects of above active components（3 dose groups ，setting up 0 μmol/L blank control group，hereinafter）on mRNA expression of key proteins JAK 2， STAT3，Akt，ERK in JAK/STAT signal pathway were measured by RT-PCR. The effects of each active component on the expression of PAI- 1，VCAM-1，ICAM-1，VEGF and NF-κB p65 were detected by ELISA. RESULTS ：Ferulic acid （6.25，25-200 μg/mL），senkyunolide H （6.25-200 μmol/L），ligustilide（200 μmol/L），cryptotanshinone（10-80 μmol/L），paeoniflorin（1.56， 6.25，12.5 μmol/L），salvianolic acid B （1.56-12.5 μmol/L，200 μmol/L）and catechin （25 μmol/L）could significantly inhibit the proliferation of HUVECs ；caffeic acid （1.56，12.5 μmol/L），ligustilide（50 μmol/L），trashinol sodium （6.25 μmol/L）and paeoniflorin（1.56，100，200 μmol/L）could significantly promote the proliferation of HUVECs （P＜0.05 or P＜0.01）. Compared with blank control group ，mRNA expression of JAK 2，STAT3 and Akt were decreased significantly in some dose groups of ferulic acid，formononetin，salvianolic acid B and astragaloside Ⅳ（P＜0.05 or P＜0.01）；the expression of PAI- 1 were significantly decreased in some dose groups of caffeic acid ，ferulic acid and n-butylphthalide；the expression of ICAM- 1 and VCAM- 1 were decreased significantly in some dose groups of caffeic acid ，ferulic acid ，n-butenylphthalide，cryptotanshinone，formononetin and catechin；the expression of NF-κB p65 were decreased significantly in some dose groups of ferulic acid ，n-butenylphthalide， formononetin，salvianolic acid B and astragaloside Ⅳ；the expression of VEGF were increased significantly in some dose groups of caffeic acid and catechin （P＜0.05 or P＜0.01）. CONCLUSIONS ：The active components of Naoxintong capsule may play the role of promoting blood circulation and removing blood stasis by inhibiting the expression of JAK/STAT signal pathway key protein mRNA and PAI- 1，ICAM-1，VCAM-1，NF-κB p65 in HUVEC ，and promoting the expression of VEGF.
