Application of metagenomic next-generation sequencing in detection of the pathogens in neonatal infectious diseases
10.3760/cma.j.cn114452-20200304-00172
- VernacularTitle:宏基因组测序技术在新生儿感染性疾病病原体检测中的应用
- Author:
Ting LIU
1
;
Hua SHI
;
Fang LIU
;
Yongmei JIANG
Author Information
1. 四川大学华西第二医院检验科 出生缺陷与相关妇儿疾病教育部重点实验室，成都 610041
- From:
Chinese Journal of Laboratory Medicine
2020;43(6):609-613
- CountryChina
- Language:Chinese
-
Abstract:
Neonatal infectious diseases (NIDs) are the main cause of neonatal death worldwide. Since the neonatal immune system is not fully developed, its pathogenic factors are more complex than adults and the clinical manifestations are more diverse. Early, rapid and comprehensive detection of infectious pathogens is of great significance for timely clinical treatment, and therefore can reduce mortality and complications. Traditional pathogen diagnosis methods are limited in small detection flux, long detection time and low screening efficiency. With the development of high-throughput sequencing technology, metagenomic next-generation sequencing (mNGS) is gradually widely applied in NIDs diagnosis. Not only can acquire the genetic information of pathogens in the samples, but also can be used to excavate the relationship between pathogens and host flora. In particular, mNGS has important reference value for the diagnosis of rare infectious diseases. In this study, the latest research progress and application of mNGS in detecting the pathogens of NIDs have been reviewed.
