Study on the NDM-1 carbapenemases producing Acinetobacter baylyi
10.3760/cma.j.cn114452-20191213-00724
- VernacularTitle:产NDM-1型碳青霉烯酶 Acinetobacter baylyi研究
- Author:
Yong LU
1
;
Yiping WANG
;
Jianfei YING
;
Yanhong YU
Author Information
1. 宁波市鄞州人民医院检验科,宁波 315040
- From:
Chinese Journal of Laboratory Medicine
2020;43(5):587-590
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the drug resistance genes carried on a carbapenem-resistant Acinetobacter spp. isolate. Methods:A retrospective study was conducted on one strain of carbapenem-resistant Acinetobacter spp, which was clinically isolated in July 2016. The strain was identified by Vitek2-compact and further confirmed by PCR detection of bla OXA-51-like, 16S-23S rRNA intergenic space sequencing and partial rpoB gene sequence analysis. Carbapenemase (NDM, KPC, VIM, IMP, SIM, SPM, GIM, OXA-23-like, OXA-24-like, OXA-58-like), 16S rRNA methylase gene (armA, rmtA, rmtB), Beta-lactamases (TEM, SHV, CTX-M, VEB, PER) and class 1 integron were amplified and detected by PCR, and plasmids were analyzed using Southern hybridization. Results:The Acinetobacter baylyi isolate showed the positive for bla NDM-1 only, and negative for the other genes. Plasmid analysis and Southern bybridization result revealed that the bla NDM-1 gene was located on unconjugatable plasmid (54 000-60 000 bp). Conclusions:Attention should be paid to the occurrence of carbapenem-resistant non-baumannii Acinetobacter spp. and the transmission mechanism of drug resistant genes.
