Detection and standardization of catecholamines and their metabolites
10.3760/cma.j.issn.1009-9158.2020.03.024
- VernacularTitle:儿茶酚胺及其代谢物的检测现状及标准化期望
- Author:
Qingxiang LIU
1
;
Weiyan ZHOU
;
Chuanbao ZHANG
Author Information
1. 北京医院 国家老年医学中心 国家卫生健康委临床检验中心 北京市临床检验工程技术研究中心 中国医学科学院老年医学研究院 中国医学科学院北京协和医学院，北京 100730
- From:
Chinese Journal of Laboratory Medicine
2020;43(3):322-327
- CountryChina
- Language:Chinese
-
Abstract:
Catecholamines include dopamine, norepinephrine and epinephrine. The main metabolites are 3-methoxytyramine, normetanephrine, metanephrine,homovanillic acid and vanillylmandelic acid. Detection of catecholamines and their metabolites is the cornerstone for the diagnosis of neuroendocrine tumors derived from neural crest such as pheochromocytoma, paraganglioma and neuroblastoma. Liquid chromatography tandem mass spectrometry has been widely used in the detection of catecholamines and their metabolites due to its high sensitivity and high specificity. However, the results of liquid chromatography tandem mass spectrometry in different laboratories are quite different and lack comparability. Accurate determination of catecholamines and their mtabolites in plasma and urine is currently a challenge in the field of clinical detectionbecause of their susceptibility to oxidative degradation, presence of numerous interferences and low concentration in plasma and urine samples.
