Brucella suis vaccine strain S2 induces apoptosis of murine BV-2 microglia cells via JNK-p53 signaling pathway

VernacularTitle:布鲁杆菌S2株通过JNK-p53信号通路诱导小鼠BV-2小胶质细胞凋亡
Author: Zhao WANG ¹ ; Boya MA ; Jiayu GUO ; Ru HE ; Yanbai WANG ; Qiang LIU ; Zhenhai WANG
Author Information

1. 宁夏医科大学临床医学院，银川　750004
From: Chinese Journal of Microbiology and Immunology 2020;40(11):857-862
CountryChina
Language:Chinese
Abstract: Objective:To investigate the potential mechanism by which Brucella suis vaccine strain S2 induces the apoptosis of BV-2 microglia cells, and to discover new protein targets for neurobrucellosis treatment. Methods:BV-2 microglia cells were treated with Brucella suis vaccine strain S2 for 0, 3, 6, 12 and 24 h. Western blot assay and RT-qPCR were performed to detect the expression of p-JNK and p53 at protein and mRNA levels in BV-2 microglia cells. Cell apoptosis was measured by flow cytometry. Immunofluorescence was used to analyze nuclear p-JNK. Results:Brucella suis vaccine strain S2 could promote the expression of p-JNK and p53 at both protein and mRNA levels and increase nuclear p-JNK in BV-2 microglia cells. Moreover, it could also induce the apoptosis of BV-2 microglia cells. Conclusions:Brucella suis vaccine strain S2 could promote the apoptosis of BV-2 microglia cells through activating JNK and promoting p53 expression.