Performance of rapid tests for HIV-1 antibody detection in paired serum and urine specimens of men who have sex with men
10.3760/cma.j.cn112309-20200628-00333
- VernacularTitle:男男性行为人群血液与尿液快速HIV-1抗体检测效力分析
- Author:
Jia LI
1
;
Ruolei XIN
;
Weidong SUN
;
Qin ZHANG
;
Shufang HE
;
Jie LI
;
Hongyan LU
Author Information
1. 北京市疾病预防控制中心 北京市预防医学研究中心 性病艾滋病防治所 100013
- From:
Chinese Journal of Microbiology and Immunology
2020;40(10):753-756
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To compare the performance of rapid tests for HIV-1 antibody detection in serum and urine specimens of men who have sex with men (MSM) for investigating suitable technology in the prevention and control of AIDS in Beijing.Methods:A total of 874 cases of MSM were recruited in the AIDS clinic of Beijing Center for Disease Prevention and Control. HIV-1/2 antibody rapid test kit (Kit A, Alere Determine), urine HIV-1 antibody rapid test kit (Kit B, Wantai Biological Pharmacy) and HIV-1/2 antibody Western blot kit (Kit C, IMT) were used for antibody detection. The sensitivity, specificity and consistency of the three rapid test kits for HIV-1 antibody detection in serum and urine specimens were analyzed.Results:Among the 874 cases of MSM, 447 were positive for HIV-1 antibody (51.14%) and 427 were negative. One false negative result occurred by using Kit A and 23 by using Kit B. Taking Kit C as reference, the sensitivity of Kit A and Kit B was 99.78% and 94.85%, respectively; the specificity of both was 100%; the overall consistency was 99.89% ( Kappa=0.998) and 97.37% ( Kappa=0.947), respectively. Conclusions:Although the sensitivity of urine rapid test kit was not as sensitive as serum rapid test kit, it was more suitable for self-test due to its convenience in sampling, high safety and high accessibility. It was suggested that urine rapid test kit should be popularized in MSM population for HIV-1 antibody screening.
