Stability and pathophysiology of volume-controlled hemorrhagic shock model in rat
10.3760/cma.j.cn113855-20200619-00492
- VernacularTitle:大鼠固定失血量失血性休克模型稳定性及病理生理变化的研究
- Author:
Panpan CHANG
1
;
Zhiwei WANG
;
Jing ZHOU
;
Wei HUANG
;
Yuanyuan ZHANG
;
Fengxue ZHU
;
Tianbing WANG
;
Baoguo JIANG
Author Information
1. 国家创伤医学中心 北京大学人民医院创伤救治中心 创伤救治与神经再生教育部重点实验室 100044
- From:
Chinese Journal of General Surgery
2020;35(9):721-725
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a rat model of volume-controlled hemorrhage and observe the pathophysiological changes that ensued.Methods:Sprague Dawley (SD) rats were subjected to femoral arterial cannulation and hemorrhage for 40% or 50% of total volume. The hemodynamics data and arterial blood gas were collected, and survival after hemorrhage was monitored for 24 hours ( n=8/group). Then lung, liver, intestine and kidney were collected for HE staining after 40% hemorrhage at 4, 8 and 12 hours ( n=6/group). Results:Mean arterial pressure of 40% and 50% blood loss group decreased significantly from (90±5)mmHg and (93±4)mmHg to (40±4)mmHg and (34±4)mmHg ( P<0.05). Heart rate of 40% and 50% blood loss group increased significantly from (330±35) bpm and (336±32) bpm to (478±36) bpm and (490±21) bpm ( P<0.05). Hemoglobin and pH decreased significantly following hemorrhage, while lactate increased. The survival rate of 50% blood loss group was just 12.5% and 40% blood loss group was 100% ( P<0.05). Compared with sham group, the most significant injury was observed in lung and small intestine after 4 hours, in liver after 8 hours and in kidney after 12 hours following hemorrhage. Conclusion:This study established a stable and repeatable volume-controlled hemorrhagic shock model in rat.
