Effect of small interfering RNA silencing high mobility group box 1 expression on the biological characteristics of hep-2/v resistant cell line of human laryngeal squamous cell carcinoma
10.3760/cma.j.cn115455-20191115-00924
- VernacularTitle:小干扰RNA干扰高迁移率族蛋白B1表达对喉癌Hep-2/v耐药细胞株生物学特性的影响
- Author:
Fangzhu LIN
1
;
Wei ZHU
;
Ping WANG
;
Delong LIU
Author Information
1. 大连市中心医院耳鼻咽喉头颈外科 116021
- From:
Chinese Journal of Postgraduates of Medicine
2020;43(4):359-364
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the effect of small interfering RNA (siRNA) silencing high mobility group box 1 (HMGB1) on the biological characteristics of hep-2/v resistant cells line of human laryngeal squamous cell carcinoma (HSCC).Methods:In Dalian Municipal Central Hospital, siRNA-HMGB1 was transfected into Hep-2/v cells. The expression of HMGB1 and MDR1 protein were detected by Western blot and cell immunofluorescence, cell proliferation activity was detected by CCK-8, and cell cycle was detected by flow cytometry.Results:Compared with that of the negative control group and the blank control group, the proliferation ability of the positive control group decreased by 20.18% at 24 h ( P<0.01) and 21.92% at 48 h ( P<0.05). The proportion of G 1 cells increased obviously. The expression of HMGB1 protein decreased 41.38% ( P<0.01), the expression of HMGB1 protein decreased 26.77% ( P<0.05). Conclusions:Reduction of HMGB1 expression in hep-2/v cells can inhibit cell proliferation and reduce drug resistance.
