Validation of Octanoate Breath Test for Measuring Gastric Emptying in Rats.
- Author:
Ingrid DEMEDTS
1
;
Christophe VANORMELINGEN
;
Hubert VAN BILLOEN
;
Tim VANUYTSEL
;
Ricard FARRE
;
Tatsuhiro MASAOKA
;
Alfons VERBRUGGEN
;
Kristien VERBEKE
;
Pieter VANDEN BERGHE
;
Jan TACK
Author Information
1. Translational Research Center for Gastrointestinal Disorders, University of Leuven, Leuven, Belgium. jan.tack@med.kuleuven.be
- Publication Type:Original Article
- Keywords:
Breath tests;
14C-octanoate;
Gastric emptying;
Rats
- MeSH:
Animals;
Atropine;
Benzethonium;
Breath Tests;
Caprylates;
Eating;
Gamma Cameras;
Gastric Emptying;
Humans;
Hydroxides;
Male;
Meals;
Rats;
Rats, Wistar;
Retention (Psychology);
Spectrum Analysis
- From:Journal of Neurogastroenterology and Motility
2013;19(2):171-178
- CountryRepublic of Korea
- Language:English
-
Abstract:
BACKGROUND/AIMS: Lack of simple and repeatable tests hampers gastric emptying studies in rats. The aim of this study was to adapt the 14C-octanoate solid gastric emptying breath test for application in rats, and to validate it against radioscintigraphic method. METHODS: After ingestion of a meal containing 3 mCi 99mTc and 2 microCi 14C-octanoate, 23 male Wistar rats were placed on a gamma camera in a airflow container. Scintigraphic images were taken at regular intervals. The amount of 14CO2 in a regularly replaced hyamine hydroxide solution, capturing CO2 in the outflow air, was counted using liquid scintillation spectrometry. 99mTc gastric retention curves and 14CO2-excretion curves were fitted to their respective data. Three rats underwent the same procedures after administration of atropine. RESULTS: Overall Tr10% (time at which 10% of the original amount of 99mTc remained in the stomach) was 355 +/- 64 minutes; Te90% (time at which 90% of total amount of 14CO2 was excreted) was 325 +/- 106 minutes. Their correlation coefficient was 0.71, R-square 0.50 and P < 0.005. Tr1/2 (50% of original amount of 99mTc remained) was 124 +/- 28 minutes; Te1/2 (50% of total amount of 14CO2 excreted) 114 +/- 32 minutes. Their correlation coefficient was 0.83 with R-square of 0.69 and P < 0.00005. In 12 immobilized animals correlation was even better: correlation coefficient 0.84; R-square 0.71 and P < 0.001 (Tr10% was 388 +/- 117 minutes; Te90% 532 +/- 219 minutes; Tr1/2 of 165 +/- 54 minutes; Te1/2 of 175 +/- 67 minutes). Atropine significantly lengthened all emptying times: 904 +/- 307 and 1461 +/- 684 minutes for Tr10% and Te90%, respectively; and 432 +/- 117 minutes for Tr1/2 and 473 +/- 190 minutes for Te1/2. CONCLUSIONS: We adapted and validated the 14C-octanoate gastric emptying breath test for application in rats.
