Cell cycling status of human cord blood CD34+ cells during ex vivo expansion is related to the level of very late antigen expression.
10.3346/jkms.2001.16.1.20
- Author:
Ju Young SEOH
1
;
Hae Young PARK
;
Wha Soon CHUNG
;
Seung Cheol KIM
;
Myong Joon HAHN
;
Kyung Hyo KIM
;
Hee Young SHIN
;
Hyo Seop AHN
;
Kwi Won PARK
;
Kyung Ha RYU
Author Information
1. Department of Microbiology, Medical Research Center, College of Medicine, Ewha Womans University, Seoul, Korea.
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
VLA-4;
Receptors, Very Late Antigen;
Cell Cycle;
Fetal Blood;
Antigens, CD34;
Ex Vivo Expansion
- MeSH:
Antigens, CD34/analysis*;
Cells, Cultured;
DNA/analysis;
Fetal Blood/cytology*;
G2 Phase;
Hematopoietic Stem Cells/physiology*;
Human;
Immunophenotyping;
Infant, Newborn;
Integrins/analysis*;
Receptors, Lymphocyte Homing/analysis*;
S Phase
- From:Journal of Korean Medical Science
2001;16(1):20-24
- CountryRepublic of Korea
- Language:English
-
Abstract:
Very late antigen-4 (VLA-4), which binds to the extracellular matrix protein fibronectin, is an integrin molecule known to be modulated during mobilization of CD34+ cells, and to be involved in signaling the mobilization stimuli. On the hypothesis that cell cycling status might be different depending on the level of VLA-4 expression, we investigated the DNA contents of human cord blood CD34+ cells during ex vivo expansion by recombinant human thrombopoietin and flt3-ligand with simultaneous measurement of surface VLA-4 at the 1st and 4th week. During this ex vivo expansion, expression of VLA-4 increased and almost all cells became VLA-4+ until the 4th day of culture. Expression of VLA-4 was maintained in the major population of the cultured cells until the 4th week. The cells in S/G2/M phase were greater in number in VLA-4 high fraction than in VLA-4 low fraction (n=4, p<.001). Furthermore, the fraction of cells in S/G2/M phase increased as the expression of VLA-4 became higher. These results suggest that cord blood CD34+ cells expressing high levels of VLA-4 have more proliferative activities.
