Expression and correlation of LC3-Ⅱ and ATF3 in hepatocellular carcinoma
10.3760/cma.j.cn121382-20200118-00305
- VernacularTitle:LC3-Ⅱ和ATF3在肝细胞癌中的表达及其相关性研究
- Author:
Yantao ZHANG
1
;
Zhijie CHU
;
Junjun SUN
;
Weifeng LIU
;
Cheng YANG
;
Yanhui YANG
Author Information
1. 河南科技大学临床医学院，洛阳 471003
- From:
International Journal of Biomedical Engineering
2020;43(3):193-198
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To analyze the expression of LC3-Ⅱ and ATF3 in hepatocellular carcinoma (HCC) , to explore the relationship between their expression and the prognosis of patients with HCC, and to analyze the expression correlation betweenLC3-Ⅱ and ATF3 in HCC tissues.Methods:Immunohistochemical method was used to detect the expression of LC3-Ⅱ and ATF3 protein in HCC tissue and corresponding paracancerous tissue specimens, to analyze the relationship between these two proteins and the patient's clinicopathological characteristics as well as survival time. Western blot was used to detect the expression of LC3-Ⅱ and ATF3 proteins in fresh HCC tissues and corresponding paracancerous tissue.Results:The expression of LC3-Ⅱ and ATF3 inparacancerous tissues was significantly higher than that in HCC tissues. The expression levels of were related to HCC histopathological grade and venous tumor thrombus (all P<0.05), but were not related to age, gender, and serum alpha-fetoprotein, tumor diameter, HBsAg, etc (all P>0.05) . The low expression of LC3-Ⅱ and ATF3 was significantly related to the poor prognosis of patients with HCC (both P<0.05). Conclusions:Both LC3-Ⅱ and ATF3 protein expression are related to the occurrence and development of HCC. The combined detection of LC3-Ⅱ and ATF3 is helpful for the assessment of the malignant degree of HCC and it is expected to become an important indicator for judging the prognosis of patients.
