Investigation of Paidu Qingzhi Tablets Based on UPLC-Q-TOF-MS and Network Pharmacology
10.13422/j.cnki.syfjx.20192351
- VernacularTitle:排毒清脂片的UPLC-Q-TOF-MS成分分析及网络药理学探索
- Author:
Ze-yun LI
1
;
Xing CAO
2
;
Yong-liang YUAN
1
;
Hao CUI
3
;
Er-qiang YANG
1
;
Xin TIAN
1
;
Xiao-jian ZHANG
1
Author Information
1. The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China
2. Jilin Medical University, Jilin 132000, China
3. Furen Pharmaceutical Group Co. Ltd., Zhengzhou 450046, China
- Publication Type:Research Article
- Keywords:
Paidu Qingzhi tablets;
methanol extract;
chemical compositions;
targets;
pathways;
disease;
network pharmacology
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2020;26(7):129-134
- CountryChina
- Language:Chinese
-
Abstract:
Objective::To identify the active constituents of Paidu Qingzhi tablets by UPLC-Q-TOF-MS technique, and to reveal its potential targets and molecular mechanisms by network pharmacology. Method::Chromatography separation was achieved on an ACQUITY UPLC HSS T3 column (2.1 mm×100 mm, 1.8 μm) with mobile phase consisted of 0.1% formic acid aqueous solution and acetonitrile for gradient elution (0-17 min, 95%-5%A; 17-17.01 min, 5%-95%A; 17.01-20 min, 95%A), the flow rate was 0.3 mL·min-1, the column temperature was 40 ℃. Data acquisition was carried out in electrospray ionization (ESI) under the negative ion mode, the scanning range was 50-1 200.Ingredients in Paidu Qingzhi tablets were identified according to reference substance, relative molecular weight, mass spectrometric cleavage rule and literature information. Multiple databases were used to retrieve the targets of these identified ingredients and related diseases treated by the tablets, and metabolic pathways. A visual network of " herbs-compounds-targets-pathways-diseases" was constructed by Cytoscape 3.7.1. Result::A total of 33 active compounds in methanol extract of Paidu Qingzhi tablets were identified by UPLC-Q-TOF-MS, including 27 compounds from Rhei Radix et Rhizoma, 4 compounds from Panacis Quinquefolii Radix, 2 compounds from Ophiopogonis Radix. Totally 18 direct targets and 58 indirect targets corresponding to 31 ingredients were obtained, 7 metabolic pathways including steroid hormone biosynthesis pathway, arachidonic acid metabolic pathway, insulin signaling pathway were strongly correlated with the treatment of hyperlipidemia, acne and simple obesity by Paidu Qingzhi tablets. Conclusion::Ingredients of Paidu Qingzhi tablets are revealed by UPLC-Q-TOF-MS, based on these identified ingredients, targets and related metabolic pathways are visualized by network pharmacology. The current research can provide theoretical basis for quality control and clinical application of Paidu Qingzhi tablets.
