Analysis of Chemical Constituents in Baihe Dihuangtang by UPLC-Q-TOF-MS
10.13422/j.cnki.syfjx.20192253
- VernacularTitle:基于UPLC-Q-TOF-MS技术分析百合地黄汤的化学成分
- Author:
Fei ZHOU
1
;
Mei-si LIN
2
;
Rui LI
3
;
Chun-rong ZHANG
3
;
Li ZHAO
3
;
Da-sheng LIN
1
;
Ke CAO
3
Author Information
1. School of Pharmacy, Key Laboratory of Traditional Chinese Medicine (TCM) Resources with Southwest Characteristics, Chengdu University of TCM, Chengdu 611137, China
2. School of Acupuncture and Moxibustion, Affiliated Hospital of Chengdu University of TCM, Chengdu 610097, China
3. Chengdu Taihe Health Technology Group Co. Ltd., Chengdu 611731, China
- Publication Type:Research Article
- Keywords:
Baihe Dihuangtang;
chemical constituents;
phenolic glycerides;
phenylethanoid glycosides;
iridoids;
lonones;
phenylpropanoids
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2020;26(7):15-22
- CountryChina
- Language:Chinese
-
Abstract:
Objective::To study the chemical constituents in Baihe Dihuangtang by ultra-performance liquid chromatography-quadrupole-time of flight-mass spectrometry (UPLC-Q-TOF-MS). Method::The separation was performed on an ACQUITY UPLC BEH C18 column (2.1 mm×50 mm, 1.7 μm) by a gradient elution of acetonitrile-0.1%formic acid solution. The flow rate was 0.4 mL·min-1 and the column temperature was 30 ℃, the injection volume was 5 μL. Electrospray ionization was applied and the data were collected via positive and negative ion modes. By using SCIEX OS 1.4 software, the chemical constituents were analyzed based on the retention time, excimer ion peak and fragment ion peak of the compounds, as well as comparison with reference substances and literature data. Result::A total of 49 chemical constituents in Baihe Dihuangtang were identified, including 5 phenolic glycerides, 15 phenylethanoid glycosides, 11 iridoids, 8 lonones, 3 phenylpropanoids, 2 nucleosides, 1 organic acid, and 4 other compounds. Among them, phenolic glycerides belonged to Lilii Bulbus, and other components mainly belonged to Rehmanniae Radix. Four chemical constituents (acteoside, isoacteoside, ferulic acid and caffeic acid) were identified by comparison of reference substances. Conclusion::The established detection method can quickly and accurately analyze the chemical constituents of Baihe Dihuangtang. The information of chemical constituents in Baihe Dihuangtang is comprehensively expounded. The study establishes a foundation for the research of quality control, material foundation of efficacy and the development of compound preparations of Baihe Dihuangtang.
