Purification of Plasmodium and Babesia- infected erythrocytes using a non-woven fabric filter
https://doi.org/10.47665/tb.37.4.911
- Author:
Tao, Z.Y.
1
;
Liu, W.P.
1
;
Dong, J.
2
;
Feng, X.X.
3
;
Yao, D.W.
3
;
Lv, Q.L.
4
;
Ibrahim, U.
1
;
Dong, J.J.
4
;
Culleton, R.
5
;
Gu, W.
4
;
Su, P.P.
4
;
Tao, L.
4
;
Li, J.Y.
4
;
Fang, Q.
1
;
Xia, H.
1
Author Information
1. Department of Microbiology and Parasitology, Bengbu Medical College, 2600# Donghai Avenue, Bengbu 233030, China&Anhui Provincial Key Laboratory of Infection and Immunology, Bengbu Medical College, 2600# Donghai Avenue, Bengbu 233030, China
2. Department of Microbiology, Bengbu Center for Disease Control and Prevention, 700# Huayuan Road, Bengbu 233000, China
3. College of Veterinary Medicine, Nanjing Agricultural University, No.1 Weigang Road, Nanjing 210095, China
4. Department of Microbiology and Parasitology, Bengbu Medical College, 2600# Donghai Avenue, Bengbu 233030, China&
5. Department of Molecular Parasitology, Proteo-Science Center, Ehime University, Shitsukawa, Toon, Ehime 791-0295, Japan
- Publication Type:Journal Article
- From:Tropical Biomedicine
2020;37(No.4):911-918
- CountryMalaysia
- Language:English
-
Abstract:
The purification of parasite-infected erythrocytes from whole blood containing
leucocytes is crucial for many downstream genetic and molecular assays in parasitology.
Current methodologies to achieve this are often costly and time consuming. Here, we
demonstrate the successful application of a cheap and simple Non-Woven Fabric (NWF) filter
for the purification of parasitized red blood cells from whole blood. NWF filtration was
applied to the malaria-parasitized blood of three strains of mice, and one strain of rat, and to
Babesia gibsoni parasitized dog blood. Before and after filtration, the white blood cell (WBC)
removal rates and red blood cell (RBC) recovery rates were measured. After NWF filter
treatment of rodent malaria-infected blood, the WBC removal rates and RBC recovery rates
were, for Kunming mice: 99.51%±0.30% and 86.12%±8.37%; for BALB/C mice: 99.61%±0.15%
and 80.74%±7.11%; for C57 mice: 99.71%±0.12% and 84.87%±3.83%; for Sprague-Dawley rats:
99.93%±0.03% and 83.30%±2.96%. Microscopy showed WBCs were efficiently removed from
infected dog blood samples, and there was no obvious morphological change of B. gibsoni
parasites. NWF filters efficiently remove leukocytes from malaria parasite-infected mouse
and rat blood, and are also suitable for filtration of B. gibsoni-infected dog blood.
- Full text:8.2020my1177.pdf
