Role of IL-1 beta(Interleukin 1 beta) on Bone Resorption in Mouse Calvarial Bone.
- Author:
Sang Ho MOON
1
;
Kyu Min KONG
;
Dong Jun KIM
;
Cheorl Ho KIM
Author Information
1. Department of Orthopedic Surgery, Handong University Sunlin Hospital, Pohang, Korea. msh@sunlin.com
- Publication Type:Original Article
- Keywords:
Interleukin-1 beta;
Osteoporosis;
PGE2;
Plasminogen activator;
Bone resorption
- MeSH:
Alkaline Phosphatase;
Animals;
Bone Resorption*;
Cell Proliferation;
Cells, Cultured;
Dinoprostone;
Interleukin-1*;
Interleukin-1beta;
Metabolism;
Mice*;
Osteoporosis;
Plasminogen Activators;
Radioimmunoassay;
Ulna
- From:Journal of Korean Orthopaedic Research Society
2004;7(2):152-158
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: To determine the interactions between interleukin-1 beta(IL-1 beta) and bone cells in a fetal mouse bone model. MATERIALS AND METHODS: Fetal mouse calvarial bone cells were isolated and cultured. Alkaline phosphatase activity was measured by monitoring the release of p-nitrophenol from disodium p-nitrophenylphosphate. PGE2 was measured by radioimmunoassay and plasminogen activator activity was measured using [125 I]-fibrin coated multi-wells. The effects of systemic and local factors on bone resorption were assessed by measuring the percentage of 45 Ca release from prelabeled 19-day-old fetal mouse radii and ulnae by radioimmunoassay after 5 days culture. RESULTS: In this study, IL-1 beta stimulated cellular proliferation and the synthesis of prostaglandin E2 and plasminogen activator activity in the cultured cells in a dose-dependent manner. but alkaline phosphatase activity was decreased by IL-1 beta in a dose-dependent manner CONCLUSION: These studies support the role of IL-1 beta in the pathological modulation of bone cell metabolism, with regard to implication in the pathogenesis of osteoporosis by IL-1 beta.
