Study on quality control of Miao medicine Laportea bulbifera based on fingerprint analysis and quantitative analysis of multi-components
10.7501/j.issn.0253-2670.2020.16.029
- VernacularTitle: 基于指纹图谱和多指标定量测定的苗药红禾麻质量控制研究
- Author:
He-Jia HU
1
Author Information
1. Provincial Key Laboratory of Pharmaceutics in Guizhou, State Key Laboratory of Functions and Applications of Medicinal Plants, Guizhou Provincial Engineering Research Center for the Development and Application of Ethnic Medicine and TCM, School of Pharmacy, Guizhou Medical University
- Publication Type:Journal Article
- Keywords:
Chlorogenic acid;
Cryptochlorogenic acid;
Epicatechin fingerprinting;
Epigallocatechin;
Isoquercitrin;
Kaempferol-3-O-rutinoside;
Laportea bulbifera (Sieb.et Zucc.) Wedd.;
Luteoloside;
Neochlorogenic acid;
Quality control;
Quercitrin;
Rutin
- From:
Chinese Traditional and Herbal Drugs
2020;51(16):4325-4330
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To establish a rational method for Laportea bulbifera quality control. Methods: The fingerprint technique and multi-component quantitation were used to study the quality control of L. bulbifera by UHPLC. The 12 batches of L. bulbifera UHPLC fingerprint were evaluated by the evaluation system on similitude degree of chromatogram fingerprint of traditional Chinese medicine. Results: The quality control methods of Miao medicine L. bulbifera for simultaneous determination of 10 components (including neochlorogenic acid, cryptochlorogenic acid, chlorogenic acid, rutin, isoquercitrin, luteoloside, kaempferol-3-O-rutinoside, quercitrin, epigallocatechin and epicatechin) were established. The linear, precision, repeatability and stability are good. The standard recoveries were 95.89%-98.62%, with RSD less than 3%. The common mode of fingerprint was established after determination fingerprints of 12 batches of samples of L. bulbifera by UHPLC. There were 20 common peaks in these samples. The similarity of the 12 batches fingerprints were in the range from 0.805 to 0.931. Conclusion: The fingerprinting and multi-index content determination methods for quantitative control of Miao medicine L. bulbifera have high sensitivity, good accuracy, stability and reliability, which can provide a theoretical and experimental foundation for quantitative control of Miao medicine L. bulbifera.
