Exosomes derived from lipopolysaccharide-preconditioned dental folic cells regulate osteogenic differentiation of periodontal ligament cell in periodontitis
10.12016/j.issn.2096⁃1456.2021.02.002
- Author:
SHI Weiwei
1
,
2
,
3
;
DING Yi
3
;
TIAN Weidong
1
,
2
,
4
;
GUO Shujuan
1
,
5
Author Information
1. Engineering Research Center of Oral Translational Medicine, Ministry of Education, Sichuan University
2. National Engineering Laboratory for Oral Regenerative Medicine, West China Hospital of Stomatology, Sichuan University
3. State Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases, Department of Periodontics, West China Hospital of Stomatology, Sichuan University
4. State Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases, Department of Oral and Maxillofacial Surgery, West China Hospital of Stomatology, Sichuan University
5. National Engineering Laboratory for Oral Regenerative Medicine, West China Hospital of Stomatology 3State Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases, Department of Periodontics, West China Hospital of Stomatology, Sichuan University.
- Publication Type:Journal Article
- Keywords:
lipopolysaccharide;
dental folic cells;
periodontal ligament cells;
exosomes;
osteogenic differentiation;
collagen fiber;
mineralization;
periodontal regeneration
- From:
Journal of Prevention and Treatment for Stomatological Diseases
2021;29(2):81-87
- CountryChina
- Language:Chinese
-
Abstract:
Objective:The purpose of this study was to investigate the effect of different concentrations of exosomes (Exos) secreted from dental folic cells (DFCs) preconditioned with lipopolysaccharide (LPS) on the osteogenic differentiation ability of periodontal cells in periodontitis (p-PDLCs) in patients to provide a basis for the prevention and treatment of periodontal disease.
Method :Tissue block and enzyme digestion methods were used to culture DFCs and p-PDLCs. Exosomes were isolated from 250 ng/mL LPS-preconditioned DFCs 24 h later. The characteristics of exosomes were detected by transmission electron microscopy, particle size analysis and Western blotting. The effects of 10 μg/mL and 100 μg/mL exosomes on the osteogenic differentiation of p-PDLCs were detected by RT-PCR and Alizarin red staining.
Results : LPS-pretreated DFC-derived exosomes (L-Exos) are vesicle-like structures with a size between 30-100 nm that positively express CD63 and Alix. Compared with the control group, exosomes significantly upregulated Periostin, Col Ⅰ, and Col Ⅲ expression at 100 μg/mL (P < 0.05), while TGF- β1 was significantly upregulated at 10 μg/mL (P < 0.01). At 7 days after osteogenic induction, mineralized nodules were significantly more abundant in the exosome group than in the control group (P < 0.01), and the results were better at a concentration of 100 μg/mL (P < 0.01).
Conclusion: 100 μg/mL L-Exos are better than 10 μg/mL L-Exos in enhancing the osteogenic differentiation ability of p-PDLCs.
- Full text:脂多糖预处理的牙囊细胞外泌体对牙周炎牙周膜细胞成骨分化的影响.pdf
