Protection of spinal cord ischemia-reperfusion injury mediated by N-methyl-D-aspartate receptors
- VernacularTitle:N-甲基-D-天冬氨酸受体介导的脊髓缺血-再灌注保护作用的研究
- Author:
Hong CHEN
1
;
Suwei CHEN
1
;
Shengyu WANG
1
;
Yan LI
1
;
Xu MENG
1
Author Information
1. Department of Cardiac Surgery, Beijing Anzhen Hospital, Capital Medical University, Beijing, 100029, P.R.China
- Publication Type:Journal Article
- Keywords:
Spinal cord ischemia-reperfusion;
K-1024;
glutamate;
neuronal nitric oxide synthase (nNOS);
N- methyl-D-aspartate (NMDA) receptor
- From:
Chinese Journal of Clinical Thoracic and Cardiovascular Surgery
2020;27(12):1460-1465
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the protective mechanism of spinal cord ischemia-reperfusion injury mediated by N-methyl-D-aspartate (NMDA) receptor. Methods A total of 42 SD rats were randomly assigned to 4 groups: a non-blocking group (n=6), a saline group (n=12), a NMDA receptor blocker K-1024 (25 mg/kg) group (n=12) and a voltage-gated Ca2+ channel blocker nimodipine (0.5 mg/kg) group (n=12). The medications were injected intraperitoneally 30 min before ischemia. The neural function was evaluated. The neuronal histologic change of spinal cord lumbar region, the release of neurotransmitter amino acids and expression of spinal cord neuronal nitric oxide synthase (nNOS) were compared. Results At 8 h after reperfusion, the behavioral score of the K-1024 group was 2.00±0.00 points, which was statistically different from those of the saline group (5.83±0.41 points) and the nimodipine group (5.00±1.00 points, P<0.05). Compared with the saline group and nimodipine group, K-1024 group had more normal motor neurons (P<0.05). There was no significant difference in glutamic acid concentration in each group at 10 min after ischemia (P=0.731). The nNOS protein expression in the K-1024 group was significantly down-regulated compared with the saline group (P<0.01). After 8 h of reperfusion, the expression of nNOS protein in the K-1024 group was significantly up-regulated compared with the saline group (P<0.05). Conclusion K-1024 plays a protective role in spinal cord ischemia by inhibiting NMDA receptor and down-regulating nNOS protein expression; during the reperfusion, K-1024 has a satisfactory protective effect on spinal cord function, structure and biological activity of nerve cells.
