Comparison of Autophagy mRNA Expression between Chronic Otitis Media With and Without Cholesteatoma
- Author:
Junyang JUNG
1
;
Su Young JUNG
;
Myung Gu KIM
;
Young Il KIM
;
Sang Hoon KIM
;
Seung Geun YEO
Author Information
- Publication Type:Original Article
- From:Journal of Audiology & Otology 2020;24(4):191-197
- CountryRepublic of Korea
- Language:English
-
Abstract:
Background and Objectives:Autophagy is known to be associated with pathogen infection. However, the expression of autophagy-related proteins has not been studied in chronic otitis media without cholesteatoma (COM) or with cholesteatoma (CholeOM). This study aimed to determine whether there is a difference between COM and CholeOM in autophagy-related gene mRNA expression.
Subjects and Methods:For 47 patients with chronic otitis media, the inflammatory tissues were classified into granulation tissue (COM) or cholesteatoma (CholeOM) according to biopsy results.
Results:PI3K mRNA expression (COM vs. CholeOM, mean±SD, 0.009±0.010 vs. 0.003±0.004; p=0.004) was lower, whereas Beclin-1 mRNA expression (0.089±0.107 vs. 0.176±0.163; p=0.034) was higher in the CholeOM group. Expression of PI3K mRNA in the CholeOM group was lower than that in the COM subgroups with presence of bacteria (0.022±0.019 vs. 0.001±0.001; p=0.001), otorrhea (0.049±0.068 vs. 0.003±0.004; p=0.004), and hearing loss over 40 dB (0.083±0.130 vs. 0.003±0.004; p=0.005).
Conclusions:The data suggested that different autophagy proteins play important roles in chronic otitis media according to the presence or absence of cholesteatoma.
