Down-regulated Reactive Oxygen Species by Heat Shock Protein 90 in 3-Hydroxykynurenine-induced SKN-SH Cell Death.
10.11637/kjpa.2004.17.3.231
- Author:
Dae Seong KIM
1
;
Myoung Woo LEE
;
Yoo Hun NOH
;
Do Yeon LEE
;
Hyun Jung LEE
;
Jae Hyung BACH
;
Jung Woo HAN
;
Kwang Gill LEE
;
Joo Hong YEO
;
Won Bok LEE
;
Sung Su KIM
Author Information
1. Department of Anatomy, College of Medicine, Chung-Ang University, Seoul, Korea. sungsu@cau.ac.kr
- Publication Type:Original Article
- Keywords:
3-Hydroxykynurenine (3HK);
Heat shock protein 90(HSP90);
Reactive oxygen species (ROS);
Caspase;
Apoptosis
- MeSH:
Acetylcysteine;
Apoptosis;
Cell Death*;
Heat-Shock Proteins*;
Hot Temperature*;
Reactive Oxygen Species*
- From:Korean Journal of Physical Anthropology
2004;17(3):231-240
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
In this present study, we show that 3HK-induced reactive oxygen species (ROS) accumulation and caspase activation lead to apoptotic cell death. Pretreatment with N-acetylcysteine (NAC), an effective antioxidant, significantly attenuated 3HK-induced apoptosis by way of a reduction of ROS accumulation and caspase activity. SKN-SN cells were protected from 3HK-induced cytotoxicity by heat shock protein (HSP). HSP90 effectively attenuated 3HK-mediated ROS accumulation and apoptosis. In addition, the protective effect of HSP90 was abolished by pretreatment with HSP90 anti-sense oligonucleotide, but not when pretreated with anti-senses for other HSPs. These results suggest that HSP90 protects SKN-SH cells from 3HK-induced cytotoxicity by reducing ROS levels and caspase activity.
