Long-Term Effects of Diesel Exhaust Particles on Airway Inflammation and Remodeling in a Mouse Model.
10.4168/aair.2016.8.3.246
- Author:
Byeong Gon KIM
1
;
Pureun Haneul LEE
;
Shin Hwa LEE
;
Young En KIM
;
Mee Yong SHIN
;
Yena KANG
;
Seong Hwan BAE
;
Min Jung KIM
;
Taiyoun RHIM
;
Choon Sik PARK
;
An Soo JANG
Author Information
1. Department of Internal Medicine, Soonchunhyang University Bucheon Hospital, Bucheon, Korea. jas877@schmc.ac.kr
- Publication Type:Original Article
- Keywords:
Chronic;
diesel exhaust particles;
airway remodeling
- MeSH:
Airway Remodeling;
Animals;
Bronchoalveolar Lavage;
Collagen;
Fibrosis;
Goblet Cells;
Hyperplasia;
Inflammation*;
Interferon-gamma;
Interleukin-10;
Interleukin-13;
Interleukin-6;
Interleukins;
Lung;
Lymphocytes;
Mice*;
Nebulizers and Vaporizers;
Neutrophils;
Plethysmography;
Pneumonia;
Ultrasonics;
Vascular Endothelial Growth Factor A;
Vehicle Emissions*
- From:Allergy, Asthma & Immunology Research
2016;8(3):246-256
- CountryRepublic of Korea
- Language:English
-
Abstract:
PURPOSE: Diesel exhaust particles (DEPs) can induce and trigger airway hyperresponsiveness (AHR) and inflammation. The aim of this study was to investigate the effect of long-term DEP exposure on AHR, inflammation, lung fibrosis, and goblet cell hyperplasia in a mouse model. METHODS: BALB/c mice were exposed to DEPs 1 hour a day for 5 days a week for 3 months in a closed-system chamber attached to a ultrasonic nebulizer (low dose: 100 microg/m3 DEPs, high dose: 3 mg/m3 DEPs). The control group was exposed to saline. Enhanced pause was measured as an indicator of AHR. Animals were subjected to whole-body plethysmography and then sacrificed to determine the performance of bronchoalveolar lavage and histology. RESULTS: AHR was higher in the DEP group than in the control group, and higher in the high-dose DEP than in the low-dose DEP groups at 4, 8, and 12 weeks. The numbers of neutrophils and lymphocytes were higher in the high-dose DEP group than in the low-dose DEP group and control group at 4, 8, and 12 weeks. The levels of interleukin (IL)-5, IL-13, and interferon-gamma were higher in the low-dose DEP group than in the control group at 12 weeks. The level of IL-10 was higher in the high-dose DEP group than in the control group at 12 weeks. The level of vascular endothelial growth factor was higher in the low-dose and high-dose DEP groups than in the control group at 12 weeks. The level of IL-6 was higher in the low-dose DEP group than in the control group at 12 weeks. The level of transforming growth factor-beta was higher in the high-dose DEP group than in the control group at 4, 8, and 12 weeks. The collagen content and lung fibrosis in lung tissue was higher in the high-dose DEP group at 8 and 12 weeks. CONCLUSIONS: These results suggest that long-term DEP exposure may increase AHR, inflammation, lung fibrosis, and goblet cell hyperplasia in a mouse model.
