Assay of unbound teicoplanin in human plasma by centrifugal ultrafiltration combined with ultra performance liquid chromatography-tandem mass spectrometry
10.12206/j.issn.1006-0111.202003184
- VernacularTitle:离心超滤结合超高效液相色谱串联质谱法测定人血浆中替考拉宁游离浓度
- Author:
Wenqian FU
1
;
Minxin ZHANG
2
;
Nannan YAO
1
;
Lili ZHANG
2
;
Hongtao SONG
1
Author Information
1. Department of Pharmacy, No. 900 Hospital of Joint Logistics Support Force of the PLA, Fuzhou 350025, China;College of Life Science and Biopharmaceutics, Shenyang Pharmaceutical University, Shenyang 110016, China.
2. Department of Pharmacy, No. 900 Hospital of Joint Logistics Support Force of the PLA, Fuzhou 350025, China.
- Keywords:
teicoplanin;
unbound concentration;
centrifugal ultrafiltration;
ultra performance liquid chromatography-tandem mass spectrometry
- From:
Journal of Pharmaceutical Practice
2020;38(6):547-551
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish an assay method for unbound teicoplanin in plasma by centrifugal ultrafiltration combined with ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). Methods Protein was removed from plasma by a Centrifree® ultrafiltration device. The ultrafiltrate was injected to determine the unbound concentration of teicoplanin. EndeadvorsilTM C18 column (1.8 μm, 50 mm×2.1 mm) was used with gradient elution of acetonitrile and 0.02 mol/L ammonium acetate solution (containing 0.1% formic acid). The detection was performed on a triple-quadrupole tandem mass spectrometer by multiple reaction monitoring (MRM)mode via electro spray ionization (ESI). Results The calibration curve of unbound teicoplanin in plasma was linear over the range of 0.10 to 8.00 μg/ml (r=0.999). The intra-assay precision and the inter-assay precision of samples didn't exceed 7.00%. The average relative recovery ratio was 97.9%, and the matrix effect factor was 0.97. The samples had good stability after being stored at room temperature for 10 h or at −20 ℃ for 15 days, and freeze-thawed 3 times (RSDs were all within 6.50%). Conclusion This method is convenient, fast, sensitive and accurate. It provided a basis for clinical development of teicoplanin unbound concentration monitoring.
