Genes associated with Wolbachia-induced cytoplasmic incompatibility in natural populations of Culex pipiens pallens: a preliminary study
10.16250/j.32.1374.2019197
- VernacularTitle:淡色库蚊自然种群沃尔巴克氏体感染致细胞质不亲和相关基因初探
- Author:
Ying-Fan WANG
1
;
Kun HE
2
;
Wei GUO
1
;
Hai-Fang WANG
3
;
Dong-Hui ZHANG
4
;
Mao-Qing GONG
1
;
Min-Jun JI
5
;
Lin CHEN
5
Author Information
1. School of Basic Medical Sciences, Nanjing Medical University, Nanjing 211166, China
2. School of Medical Imaging, Nanjing Medical University, China
3. Shandong Academy of Medical Sciences, Shandong Institute of Parasitic Diseases, China
4. School of International Education, Nanjing Medical University, China
5. Department of Pathogenic Biology, Nanjing Medical University, Jiangsu Provincial Key Laboratory of Modern Pathogenic Biology, China
- Publication Type:Journal Article
- Keywords:
Culex pipiens pallens;
Wolbachia;
Cytoplasmic incompatibility;
WD0513 gene
- From:
Chinese Journal of Schistosomiasis Control
2020;32(5):517-521
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the genes involved in Wolbachia-induced cytoplasmic incompatibility among three natural populations of Culex pipiens pallens in eastern China, so as to provide insights into the development of preventive and control measures for mosquito-borne diseases based on Wolbachia. Methods The cytoplasmic incompatibility was tested among three natural populations of C. pipiens pallens collected from Nanjing and Wuxi of Jiangsu Province and Tangkou of Shandong Province using reciprocal crosses. Wolbachia infection was detected in C. pipiens pallens using a PCR assay, and the expression of Wolbachia wsp and WD0513 genes was quantified using a fluorescent quantitative real-time PCR (qPCR) assay. Results Bidirectional compatibility was found between the natural populations of C. pipiens pallens collected from Nanjing and Wuxi of Jiangsu Province (t = 0.57 and 0.15, both P values > 0.05), while bidirectional incompatibility was seen between the natural populations of C. pipiens pallens collected from Tangkou of Shandong Province and Wuxi of Jiangsu Province (t = 63.81 and 43.51, both P values < 0.01), and between the natural populations of C. pipiens pallens collected from Nanjing of Jiangsu Province and Tangkou of Shandong Province (t = 39.62 and 43.12, both P values < 0.01). Wolbachia wsp gene was amplified in all three natural populations of C. pipiens pallens, and qPCR assay detected no significant difference in the Wolbachia wsp gene expression among the three natural populations of C. pipiens pallens (F = 2.15, P > 0.05). In addition, there was no significant difference in the WD0513 gene expression between the natural populations of C. pipiens pallens collected from Tangkou of Shandong Province and Nanjing of Jiangsu Province (q = 8.42, P < 0.05) or between the natural populations of C. pipiens pallens collected from Tangkou of Shandong Province and Wuxi of Jiangsu Province (q = 7.84, P < 0.05); however, there was a significant difference detected in the WD0513 gene expression between the natural populations of C. pipiens pallens collected from Nanjing and Wuxi of Jiangsu Province (q = 0.40, P > 0.05). Conclusions Different Wolbachia numbers are detected in natural populations of C. pipiens pallens collected from Nanjing and Wuxi of Jiangsu Province and Tangkou of Shandong Province, and WD0513 gene may be involved in the Wolbachia-induced cytoplasmic incompatibility among three natural populations of C. pipiens pallens.
