Bioinformatics analysis of safflower WD40 transcription factor family genes.
10.19540/j.cnki.cjcmm.20200506.107
- Author:
Gang WANG
1
;
Zheng-Ren ZHANG
1
;
Yi-Fei WANG
1
;
Ying-Qi HONG
1
;
Xiu-Ming LIU
2
;
Na YAO
2
;
Yuan-Yuan DONG
2
;
Hai-Yan LI
2
Author Information
1. College of Life Science, Jilin Agricultural University Changchun 130118, China.
2. College of Life Science, Jilin Agricultural University Changchun 130118, China Bioreactor and Drug Development, Ministry of Education, Jilin Agricultural University Changchun 130118, China.
- Publication Type:Journal Article
- Keywords:
WD40 gene;
bioinformatics analysis;
expression analyses;
phylogeny;
safflower
- MeSH:
Carthamus tinctorius;
Computational Biology;
Gene Expression Profiling;
Gene Expression Regulation, Plant;
Genome, Plant;
Multigene Family;
Phylogeny;
Plant Proteins;
genetics;
Transcription Factors;
genetics
- From:
China Journal of Chinese Materia Medica
2020;45(14):3432-3440
- CountryChina
- Language:Chinese
-
Abstract:
The WD40 transcription factor family is a gene superfamily widely found in eukaryotes, which is closely related to plant growth and development regulation. It has been reported that the WD40 transcription factor was involved in the synthesis of anthocyanins, which is one of the vital components of safflower flavonoid compounds. In this study, 40 CtWD40 members in the safflower genome were identified though bioinformatics tools and gene expression analysis methods. According to the WD40 protein sequence and phylogenetic characteristics of Arabidopsis and other plants, the safflower CtWD40 family was classified into 7 subfamilies. Conservative motif analysis was used to reveal the specific conserved motifs and gene structures of each subfamily member, and there exist a certain degree of similarities in the conserved motifs and gene structure between the closely related family members. Subsequently, the search for cis-acting elements of gene promoters found CtWD40-specific promoter elements, revealing the metabolic pathways which may involve. Next, enrichment of function analysis was employed to analyze the functional categories and cellular localization of the CtWD40 protein. Furthermore, the interactions between CtWD40 proteins predicted its potential regulatory function. Finally, 19 members of the safflower CtWD40 subfamily were analyzed by qRT-PCR, the result showed the expression patterns of these members were different in diverse tissue and flowering period. This study provides a basis for the functional and expression research of the CtWD40 genes.
