Analysis of carnosic acid metabolites in rats by UHPLC-Q-Exactive MS.
10.19540/j.cnki.cjcmm.20200519.201
- Author:
Tian-Yu LOU
1
;
Bei-Bei MA
1
;
Yao-Yue LIANG
1
;
Chen-Xiao WANG
1
;
Jin-Hui LIU
1
;
Rui-Ji LI
1
;
Shang-Yue YU
1
;
Zhi-Bin WANG
2
Author Information
1. School of Chinese Pharmacy, Beijing University of Chinese Medicine Beijing 100102, China.
2. Beijing Institution of Tongrentang Beijing 100079, China.
- Publication Type:Journal Article
- Keywords:
UHPLC-Q-Exactive MS;
carnosic acid;
metabolic pathways;
metabolites
- MeSH:
Abietanes;
Animals;
Chromatography, High Pressure Liquid;
Mass Spectrometry;
Rats;
Solid Phase Extraction
- From:
China Journal of Chinese Materia Medica
2020;45(16):3952-3960
- CountryChina
- Language:Chinese
-
Abstract:
A method of ultra-high performance liquid chromatography coupled with quadrupole/electrostatic field Obitrap high-resolution mass spectrometry(UHPLC-Q-Exactive MS) was established to comprehensively identify the metabolites of carnosic acid in rats. After oral gavage of carnosic acid CMC-Na suspension in rats, urine, plasma and feces samples were collected and pretreated by solid phase extraction(SPE). Acquity UPLC BEH C_(18 )column(2.1 mm×100 mm, 1.7 μm) was used with 0.1% formic acid solution(A)-acetonitrile(B) as the mobile phase for the gradient elution. Biological samples were analyzed by quadrupole/electrostatic field Obitrap high-resolution mass spectrometry in positive and negative ion mode. Based on the accurate molecular mass, fragment ion information, and related literature reports, a total of 28 compounds(including carnosic acid) were finally identified in rat samples. As a result, the main metabolic pathways of carnosic acid in rats are oxidation, hydroxylation, methylation, glucuronide conjugation, sulfate conjugation, S-cysteine conjugation, glutathione conjugation, demethylation, decarbonylation and their composite reactions. The study showed that the metabolism of carnosic acid in rats could be efficiently and comprehensively clarified by using UHPLC-Q-Exactive MS, providing a reference for clarifying the material basis and metabolic mechanism of carnosic acid.
