Identification of key enzyme genes involved in biosynthesis of steroidal saponins and analysis of biosynthesis pathway in Polygonatum cyrtonema.
10.19540/j.cnki.cjcmm.20200329.108
- Author:
Chun-Miao SHAN
1
;
Chen-Kai WANG
1
;
Yuan-Yuan SHI
1
;
Sheng-Xiang ZHANG
1
;
Li-Qiang ZHAO
1
;
Jia-Wen WU
2
Author Information
1. Graduate School, Anhui University of Chinese Medicine Hefei 230012, China Key Laboratory of Xin'an Medicine,Ministry of Education, Experimental Center for Scientific Research, Anhui University of Chinese Medicine Hefei 230038, China.
2. Key Laboratory of Xin'an Medicine,Ministry of Education, Experimental Center for Scientific Research, Anhui University of Chinese Medicine Hefei 230038, China Anhui Academy of Chinese Medicine Hefei 230012, China Synergetic Innovation Center of Anhui Authentic Chinese Medicine Quality Improvement Hefei 230012, China.
- Publication Type:Journal Article
- Keywords:
Polygonatum cyrtonema;
RNA sequencing;
cycloartenol synthase;
differentially expressed genes;
steroidal saponins
- MeSH:
Biosynthetic Pathways;
Gene Expression Profiling;
Polygonatum;
Saponins;
Sequence Analysis, RNA;
Transcriptome
- From:
China Journal of Chinese Materia Medica
2020;45(12):2847-2857
- CountryChina
- Language:Chinese
-
Abstract:
Steroidal saponins, which are the characteristic and main active constituents of Polygonatum, exhibit a broad range of pharmacological functions, such as regulating blood sugar, preventing cardiovascular and cerebrovascular diseases and anti-tumor. In this study, we performed RNA sequencing(RNA-Seq) analysis for the flowers, leaves, roots, and rhizomes of Polygonatum cyrtonema using the BGISEQ-500 platform to understand the biosynthesis pathway of steroidal saponins and study their key enzyme genes. The assembly of transcripts for four tissues generated 129 989 unigenes, of which 88 958 were mapped to several public databases for functional annotation, 22 813 unigenes were assigned to 53 subcategories and 64 877 unigenes were annotated to 136 pathways in KEGG database. Furthermore, 502 unigenes involved in the biosynthesis pathway of steroidal saponins were identified, of which 97 unigenes encoding 12 key enzymes. Cycloartenol synthase, the first key enzyme in the pathway of phytosterol biosynthesis, showed conserved catalytic domain and substrate binding domain based on sequence analysis and homology modeling. Differentially expressed genes(DEGs) were identified in rhizomes as compared to other tissues(flowers, leaves or roots).The 2 437 unigenes annotated by KEGG showed rhizome-specific expression, of which 35 unigenes involved in the biosynthesis of steroidal saponins. Our results greatly extend the public transcriptome dataset of Polygonatum and provide valuable information for the identification of candidate genes involved in the biosynthesis of steroidal saponins and other important secondary metabolites.
