Simultaneous rapid detection of ten stilbenes in serum of mice by UPLC-MS/MS.
10.19540/j.cnki.cjcmm.20200222.201
- Author:
Shuang-Shuang LIU
1
;
Qing LIU
1
;
Pei LI
1
;
Zhi-Hong YANG
2
;
Chun-Nian HE
1
;
Pei-Gen XIAO
1
Author Information
1. Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College Beijing 100193, China Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education Beijing 100193, China.
2. Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College Beijing 100193, China.
- Publication Type:Journal Article
- Keywords:
UPLC-MS/MS;
content determination;
method validation;
peony seed coats;
stilbenes
- MeSH:
Animals;
Chromatography, High Pressure Liquid;
Chromatography, Liquid;
Mice;
Paeonia;
Reproducibility of Results;
Seeds;
chemistry;
Stilbenes;
analysis;
Tandem Mass Spectrometry
- From:
China Journal of Chinese Materia Medica
2020;45(9):2180-2185
- CountryChina
- Language:Chinese
-
Abstract:
Stilbenes is a class of natural polyphenols with 1,2-diphenylethylene as the skeleton structure which have structural and active diversity. However, there are fewer studies on their metabolic process, which limits the in-depth research and development of such components. An UPLC-MS/MS method simultaneously determining contents of ten stilbenes was firstly established in this study and applied to study the ten stilbenes of peony seed coats in the serum of C57 mice.Piceatannol was the internal standard, and methanol was used for protein precipitation, UPLC-MS/MS with negative ion mode was used for analysis, and the method was validated.The serum samples were collected and detected after mice being oral administered with 800 mg·kg~(-1) peony seed coat extracts for 8 weeks. The results showed that suffruticosol A, suffruticosol B, suffruticosol C, trans-ε-viniferin, cis-gnetin H, trans-suffruticosol D and trans-gnetin H were detected in serum samples, and the highest is suffruticosol A. The method is simple and quick with high specificity and sensitivity, and it is suitable for quantitative determination of ten stilbenes in the serum of mice.
