Molecular cloning and functional characterization of multiple ApOSCs from Andrographis paniculata.
10.1016/S1875-5364(20)60004-8
- Author:
Jian WANG
1
;
Hui-Xin LIN
2
;
Huan ZHAO
3
;
Juan GUO
4
;
Ping SU
4
;
Jian YANG
4
;
Xiao-Yi WU
5
;
Lu-Qi HUANG
6
,
7
;
Wei GAO
8
Author Information
1. School of Traditional Chinese Medicine, Shenyang Pharmaceutical University, Shenyang 100016, China; State Key Laboratory Breeding Base of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China; School of Traditional Chinese Medicine, Capital Medical University, Beijing 100069, China.
2. Geneis (Beijing) Co., Ltd, Beijing 100102, China.
3. State Key Laboratory Breeding Base of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China; School of Traditional Chinese Medicine, Capital Medical University, Beijing 100069, China.
4. State Key Laboratory Breeding Base of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
5. School of Traditional Chinese Medicine, Capital Medical University, Beijing 100069, China.
6. School of Traditional Chinese Medicine, Shenyang Pharmaceutical University, Shenyang 100016, China; State Key Laboratory Breeding Base of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China. Electronic address: huangluqi01@
7. com.
8. State Key Laboratory Breeding Base of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China; School of Traditional Chinese Medicine, Capital Medical University, Beijing 100069, China. Electronic address: weigao@ccmu.edu.cn.
- Publication Type:Journal Article
- Keywords:
2, 3-Oxidosqualene cyclases;
A. paniculata;
Triterpenoids;
α-Amyrin;
β-Amyrin
- From:
Chinese Journal of Natural Medicines (English Ed.)
2020;18(9):659-665
- CountryChina
- Language:English
-
Abstract:
Triterpenoids have been described in Andrographis paniculata. Oleanolic acid exhibits high biological activity and is widely used in the clinic, and β-sitosterol not only has good biological activity but also plays an important physiological role in plants. However, analysis of the biosynthetic pathway of triterpenoids in Andrographis paniculata has not been reported. Here, we provide the first report of the isolation and identification of nine 2, 3-oxidosqualene cyclases (ApOSC3 to ApOSC11) from A. paniculata. The results showed that ApOSC4 represented a monofunctional synthase that could convert 2, 3-oxidosqualene to β-amyrin. ApOSC5 as a bifunctional 2, 3-oxidosqualene cyclases, could transfer 2, 3-oxidosqualene to β-amyrin and α-amyrin. ApOSC6 to ApOSC8 composed the multifunctional 2, 3-oxidosqualene cyclases that could convert 2, 3-oxidosqualene to β-amyrin, α-amyrin and one or two undetermined triterpenoids. This study provides a better understanding of the biosynthetic pathway of triterpenoids in A. paniculata, and the discovery of multifunctional 2, 3-oxidosqualene cyclases ApOSC5 to ApOSC8 of the facilitates knowledge of the compounds diversity in A. paniculata.
