Differences of B cells, plasma cells, and related cytokines expression in gingival tissues between periodontitis and periodontal healthy subjects.

Yu-Ling OUYANG; Sheng CHEN; Bin CHEN

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Differences of B cells, plasma cells, and related cytokines expression in gingival tissues between periodontitis and periodontal healthy subjects.

Author: Yu-Ling OUYANG ¹ ; Sheng CHEN ² ; Bin CHEN ³
Author Information

1. Dept. of Periodontology, Shanghai Jing-an Dental Clinic, Shanghai 200040, China.
2. Dept. of Pathology, Nanjing Stomatological Hospital, Medical School of Nanjing University, Nanjing 210008, China.
3. Dept. of Periodontology, Nanjing Stomatological Hospital, Medical School of Nanjing University, Nanjing 210008, China.
Publication Type:Journal Article
Keywords: B cell activating factor; B cells; periodontitis; plasma cells; receptor activator of nuclear factor-κB ligand
MeSH: B-Lymphocytes; Cytokines; Gingival Crevicular Fluid; Healthy Volunteers; Humans; Periodontitis; Plasma Cells
From: West China Journal of Stomatology 2020;38(3):250-255
CountryChina
Language:Chinese
Abstract: OBJECTIVE:This study aimed to compare the differences of B cells, plasma cells, and related cytokines expression in gingival tissues between periodontitis and periodontal healthy subjects.
METHODS:Gingival tissues were collected from periodontal healthy subjects (periodontal healthy group, n=12) and periodontitis patients (periodontitis group, n=15). Hematoxylin-eosin (HE) staining was used for histopathological examination. Immunohistochemical staining (CD19, CD38, and CD138) was applied to detect the expression of B cells and plasma cells. B cell-activating factor (BAFF) and soluble receptor activator of nuclear factor-κB ligand (sRANKL) were detected by enzyme-linked immunosorbent assay.
RESULTS:Extensive inflam-matory cell infiltration was found in the gingival tissues of the periodontitis group. The number of CD19(+), CD38(+), and CD138(+) cells of the periodontitis group was significantly higher than that of the periodontal healthy group (P<0.000 1). BAFF and sRANKL levels of the periodontitis group were higher than those of the periodontal healthy group (P<0.01, P< 0.001, respectively).
CONCLUSIONS:The expression of B cells, plasma cells, and their related BAFF and sRANKL cytokines were significantly higher in periodon-titis patients than those in the periodontal healthy subjects, sug-gesting that B cells and plasma cells may be involved in the development of periodontitis.